MTOR regulates lysosomal ATP-sensitive two-pore Na+ channels to adapt to metabolic state

Chunlei Cang, Yandong Zhou, Betsy Navarro, Young Jun Seo, Kimberly Aranda, Lucy Shi, Shyuefang Battaglia-Hsu, Itzhak Nissim, David E. Clapham, Dejian Ren

Research output: Contribution to journalArticlepeer-review

212 Scopus citations

Abstract

Survival in the wild requires organismal adaptations to the availability of nutrients. Endosomes and lysosomes are key intracellular organelles that couple nutrition and metabolic status to cellular responses, but how they detect cytosolic ATP levels is not well understood. Here, we identify an endolysosomal ATP-sensitive Na+ channel (lysoNaATP). The channel is a complex formed by two-pore channels (TPC1 and TPC2), ion channels previously thought to be gated by nicotinic acid adenine dinucleotide phosphate (NAADP), and the mammalian target of rapamycin (mTOR). The channel complex detects nutrient status, becomes constitutively open upon nutrient removal and mTOR translocation off the lysosomal membrane, and controls the lysosome's membrane potential, pH stability, and amino acid homeostasis. Mutant mice lacking lysoNaATP have much reduced exercise endurance after fasting. Thus, TPCs make up an ion channel family that couples the cell's metabolic state to endolysosomal function and are crucial for physical endurance during food restriction.

Original languageEnglish (US)
Pages (from-to)778-790
Number of pages13
JournalCell
Volume152
Issue number4
DOIs
StatePublished - Feb 14 2013

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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