Multi-image particle tracking velocimetry of the microcirculation using fluorescent nanoparticles

Dino J. Ravnic, Yu Zhong Zhang, Akira Tsuda, Juan P. Pratt, Harold T. Huss, Steven J. Mentzer

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Particle tracking velocimetry provides a Lagrangian description of flow properties in the microcirculation. To determine the utility of fluorescent nanoparticles to provide Lagrangian coordinates, we tracked these particles both in vitro and in vivo. The particles had a neutral charge and fluorescence intensity greater than 1000 times the PKH26-labeled red blood cells. At image acquisition rates of 60 frames per second, particles were tracked at velocities up to 4000 μm/s. Morphometric changes reflecting streaking artifact were significant at velocities of 4000 μm/s (P < 0.05), but not at lower velocities (P > 0.05). Intravital microscopy monitoring after intravenous injection of the particles demonstrated a circulation half-life that was inversely related to particle size: 500 nm nanoparticles demonstrated a smaller change in plasma concentration than larger particles. Regardless of the size of the particles, more than 50% of the recovered fluorescence was located in the liver. These results suggest that fluorescent nanoparticles provide a convenient and practical Lagrangian description of flow velocity in the microcirculation.

Original languageEnglish (US)
Pages (from-to)27-33
Number of pages7
JournalMicrovascular Research
Volume72
Issue number1-2
DOIs
StatePublished - Jul 1 2006

Fingerprint

Microcirculation
Rheology
Velocity measurement
Nanoparticles
Particle Size
Fluorescence
Image acquisition
Intravenous Injections
Flow velocity
Liver
Artifacts
Half-Life
Blood
Erythrocytes
Particle size
Cells
Plasmas
Monitoring

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Ravnic, Dino J. ; Zhang, Yu Zhong ; Tsuda, Akira ; Pratt, Juan P. ; Huss, Harold T. ; Mentzer, Steven J. / Multi-image particle tracking velocimetry of the microcirculation using fluorescent nanoparticles. In: Microvascular Research. 2006 ; Vol. 72, No. 1-2. pp. 27-33.
@article{df3f434618fd40b1b82dbbb92a098a17,
title = "Multi-image particle tracking velocimetry of the microcirculation using fluorescent nanoparticles",
abstract = "Particle tracking velocimetry provides a Lagrangian description of flow properties in the microcirculation. To determine the utility of fluorescent nanoparticles to provide Lagrangian coordinates, we tracked these particles both in vitro and in vivo. The particles had a neutral charge and fluorescence intensity greater than 1000 times the PKH26-labeled red blood cells. At image acquisition rates of 60 frames per second, particles were tracked at velocities up to 4000 μm/s. Morphometric changes reflecting streaking artifact were significant at velocities of 4000 μm/s (P < 0.05), but not at lower velocities (P > 0.05). Intravital microscopy monitoring after intravenous injection of the particles demonstrated a circulation half-life that was inversely related to particle size: 500 nm nanoparticles demonstrated a smaller change in plasma concentration than larger particles. Regardless of the size of the particles, more than 50{\%} of the recovered fluorescence was located in the liver. These results suggest that fluorescent nanoparticles provide a convenient and practical Lagrangian description of flow velocity in the microcirculation.",
author = "Ravnic, {Dino J.} and Zhang, {Yu Zhong} and Akira Tsuda and Pratt, {Juan P.} and Huss, {Harold T.} and Mentzer, {Steven J.}",
year = "2006",
month = "7",
day = "1",
doi = "10.1016/j.mvr.2006.04.006",
language = "English (US)",
volume = "72",
pages = "27--33",
journal = "Microvascular Research",
issn = "0026-2862",
publisher = "Academic Press Inc.",
number = "1-2",

}

Multi-image particle tracking velocimetry of the microcirculation using fluorescent nanoparticles. / Ravnic, Dino J.; Zhang, Yu Zhong; Tsuda, Akira; Pratt, Juan P.; Huss, Harold T.; Mentzer, Steven J.

In: Microvascular Research, Vol. 72, No. 1-2, 01.07.2006, p. 27-33.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Multi-image particle tracking velocimetry of the microcirculation using fluorescent nanoparticles

AU - Ravnic, Dino J.

AU - Zhang, Yu Zhong

AU - Tsuda, Akira

AU - Pratt, Juan P.

AU - Huss, Harold T.

AU - Mentzer, Steven J.

PY - 2006/7/1

Y1 - 2006/7/1

N2 - Particle tracking velocimetry provides a Lagrangian description of flow properties in the microcirculation. To determine the utility of fluorescent nanoparticles to provide Lagrangian coordinates, we tracked these particles both in vitro and in vivo. The particles had a neutral charge and fluorescence intensity greater than 1000 times the PKH26-labeled red blood cells. At image acquisition rates of 60 frames per second, particles were tracked at velocities up to 4000 μm/s. Morphometric changes reflecting streaking artifact were significant at velocities of 4000 μm/s (P < 0.05), but not at lower velocities (P > 0.05). Intravital microscopy monitoring after intravenous injection of the particles demonstrated a circulation half-life that was inversely related to particle size: 500 nm nanoparticles demonstrated a smaller change in plasma concentration than larger particles. Regardless of the size of the particles, more than 50% of the recovered fluorescence was located in the liver. These results suggest that fluorescent nanoparticles provide a convenient and practical Lagrangian description of flow velocity in the microcirculation.

AB - Particle tracking velocimetry provides a Lagrangian description of flow properties in the microcirculation. To determine the utility of fluorescent nanoparticles to provide Lagrangian coordinates, we tracked these particles both in vitro and in vivo. The particles had a neutral charge and fluorescence intensity greater than 1000 times the PKH26-labeled red blood cells. At image acquisition rates of 60 frames per second, particles were tracked at velocities up to 4000 μm/s. Morphometric changes reflecting streaking artifact were significant at velocities of 4000 μm/s (P < 0.05), but not at lower velocities (P > 0.05). Intravital microscopy monitoring after intravenous injection of the particles demonstrated a circulation half-life that was inversely related to particle size: 500 nm nanoparticles demonstrated a smaller change in plasma concentration than larger particles. Regardless of the size of the particles, more than 50% of the recovered fluorescence was located in the liver. These results suggest that fluorescent nanoparticles provide a convenient and practical Lagrangian description of flow velocity in the microcirculation.

UR - http://www.scopus.com/inward/record.url?scp=33746330742&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746330742&partnerID=8YFLogxK

U2 - 10.1016/j.mvr.2006.04.006

DO - 10.1016/j.mvr.2006.04.006

M3 - Article

C2 - 16806290

AN - SCOPUS:33746330742

VL - 72

SP - 27

EP - 33

JO - Microvascular Research

JF - Microvascular Research

SN - 0026-2862

IS - 1-2

ER -