Multi-step excitation energy transfer engineered in genetic fusions of natural and synthetic light-harvesting proteins

Joshua A. Mancini, Goutham Kodali, Jianbing Jiang, Kanumuri Ramesh Reddy, Jonathan S. Lindsey, Donald A. Bryant, P. Leslie Dutton, Christopher C. Moser

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Synthetic proteins designed and constructed from first principles with minimal reference to the sequence of any natural protein have proven robust and extraordinarily adaptable for engineering a range of functions. Here for the first time we describe the expression and genetic fusion of a natural photosynthetic light-harvesting subunit with a synthetic protein designed for light energy capture andmulti-step transfer.We demonstrate excitation energy transfer fromthe bilin of the CpcA subunit (phycocyanin a subunit) of the cyanobacterial photosynthetic light-harvesting phycobilisome to synthetic four-helix-bundle proteins accommodating sites that specifically bind a variety of selected photoactive tetrapyrroles positioned to enhance energy transfer by relay. The examination of combinations of different bilin, chlorin and bacteriochlorin cofactors has led to identification of the preconditions for directing energy from the bilin light-harvesting antenna into synthetic protein-cofactor constructs that can be customized for light-activated chemistry in the cell.

Original languageEnglish (US)
Article number20160896
JournalJournal of the Royal Society Interface
Volume14
Issue number127
DOIs
StatePublished - Feb 1 2017

Fingerprint

Excitation energy
Energy Transfer
Energy transfer
Bile Pigments
Fusion reactions
Proteins
Light
Phycobilisomes
Tetrapyrroles
Phycocyanin
Antennas

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering

Cite this

Mancini, Joshua A. ; Kodali, Goutham ; Jiang, Jianbing ; Reddy, Kanumuri Ramesh ; Lindsey, Jonathan S. ; Bryant, Donald A. ; Dutton, P. Leslie ; Moser, Christopher C. / Multi-step excitation energy transfer engineered in genetic fusions of natural and synthetic light-harvesting proteins. In: Journal of the Royal Society Interface. 2017 ; Vol. 14, No. 127.
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Multi-step excitation energy transfer engineered in genetic fusions of natural and synthetic light-harvesting proteins. / Mancini, Joshua A.; Kodali, Goutham; Jiang, Jianbing; Reddy, Kanumuri Ramesh; Lindsey, Jonathan S.; Bryant, Donald A.; Dutton, P. Leslie; Moser, Christopher C.

In: Journal of the Royal Society Interface, Vol. 14, No. 127, 20160896, 01.02.2017.

Research output: Contribution to journalArticle

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