Multi-Virulence-Locus Sequence Typing of Listeria monocytogenes

Wei Zhang, Bhushan M. Jayarao, Stephen J. Knabel

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

A multi-virulence-locus sequence typing (MVLST) scheme was developed for subtyping Listeria monocytogenes, and the results obtained using this scheme were compared to those of pulsed-field gel electrophoresis (PFGE) and the published results of other typing methods, including ribotyping (RT) and multilocus sequence typing (MLST). A set of 28 strains (eight different serotypes and three known genetic lineages) of L. monocytogenes was selected from a strain collection (n > 1,000 strains) to represent the genetic diversity of this species. Internal fragments (ca. 418 to 469 bp) of three virulence genes (prfA, inlB, and inlC) and three virulence-associated genes (dal, lisR, and clpP) were sequenced and analyzed. Multiple DNA sequence alignment identified 10 (prfA), 19 (inlB), 13 (dal), 10 (lisR), 17 (inlC), and 16 (clpP) allelic types and a total of 28 unique sequence types. Comparison of MVLST with automated EcoRI-RT and PFGE with ApaI enzymatic digestion showed that MVLST was able to differentiate strains that were indistinguishable by RT (13 ribotypes; discrimination index = 0.921) or PFGE (22 profiles; discrimination index = 0.970). Comparison of MVLST with housekeeping-gene-based MLST analysis showed that MVLST provided higher discriminatory power for serotype 1/2a and 4b strains than MLST. Cluster analysis based on the intragenic sequences of the selected virulence genes indicated a strain phylogeny closely related to serotypes and genetic lineages. In conclusion, MVLST may improve the discriminatory power of MLST and provide a convenient tool for studying the local epidemiology of L. monocytogenes.

Original languageEnglish (US)
Pages (from-to)913-920
Number of pages8
JournalApplied and environmental microbiology
Volume70
Issue number2
DOIs
StatePublished - Feb 1 2004

Fingerprint

Listeria monocytogenes
virulence
Virulence
Multilocus Sequence Typing
loci
Ribotyping
Pulsed Field Gel Electrophoresis
pulsed-field gel electrophoresis
DNA fingerprinting
electrokinesis
serotypes
gel
gene
Genes
ribotypes
genes
Sequence Alignment
Essential Genes
sequence alignment
Phylogeny

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

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Multi-Virulence-Locus Sequence Typing of Listeria monocytogenes. / Zhang, Wei; Jayarao, Bhushan M.; Knabel, Stephen J.

In: Applied and environmental microbiology, Vol. 70, No. 2, 01.02.2004, p. 913-920.

Research output: Contribution to journalArticle

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