Multicolor fluorescence detection on an electrophoretic microdevice using an acoustooptic tunable filter

James M. Karlinsey, James P. Landers

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

An acoustooptic tunable filter (AOTF) is used to detect multiple fluorescent signals on a fluidic microdevice. A confocal laser-induced fluorescence detection setup is used to excite fluorescent dyes in glass microchannels, presenting a streamlined and robust detection system consisting of the narrow-bandwidth AO filter and a single photodetector. The flexibility of the filter is demonstrated by alternating between wavelengths for precise microchannel alignment and sweeping through a range of wavelengths for preliminary spectral characterization of subnanoliter probe volumes of target analytes. The AOTF is also coupled with an electrophoretic separation for the multicolor detection of PCR-amplified DNA against a labeled sizing standard, the discrimination of multiple amplicons overlapped in time, and the identification of amplified biowarfare agents in a fluorescent spiking experiment. Finally, to demonstrate the multicolor capability of the system, 19-wavelength detection is performed during the separation of a three-dye sample mixture.

Original languageEnglish (US)
Pages (from-to)5590-5596
Number of pages7
JournalAnalytical chemistry
Volume78
Issue number15
DOIs
StatePublished - Aug 1 2006

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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