Multiframe particle tracking in intravital imaging: Defining Lagrangian coordinates in the microcirculation

Dino J. Ravnic, Akira Tsuda, Aslihan Turhan, Juan P. Pratt, Harold T. Huss, Yu Zhong Zhang, Steven J. Mentzer

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The cellular composition of the microcirculation creates blood flow that can be unsteady and nonuniform. To obtain information about nonuniform cellular trajectories, we describe in vivo imaging techniques that provide both detailed tracking of individual particles as well as an approach to simultaneous multicolor particle tracking. Particularly relevant to biologic systems, Lagrangian methods provide information about the fate of individual particles and flow in the system.

Original languageEnglish (US)
Pages (from-to)597-601
Number of pages5
JournalBioTechniques
Volume41
Issue number5
DOIs
StatePublished - Nov 2006

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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