Multifunctional, micropipette-based method for incorporation and stimulation of bacterial mechanosensitive ion channels in droplet interface bilayers

Joseph S. Najem, Myles D. Dunlap, Anthony Yasmann, Eric C. Freeman, John W. Grant, Sergei Sukharev, Donald J. Leo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

MscL, a large conductance mechanosensitive channel (MSC), is a ubiquitous osmolyte release valve that helps bacteria survive abrupt hypoosmotic shocks. It has been discovered and rigorously studied using the patch-clamp technique for almost three decades. Its basic role of translating tension applied to the cell membrane into permeability response makes it a strong candidate to function as a mechanoelectrical transducer in artificial membrane-based biomolecular devices. Serving as building blocks to such devices, droplet interface bilayers (DIBs) can be used as a new platform for the incorporation and stimulation of MscL channels. Here, we describe a micropipette-based method to form DIBs and measure the activity of the incorporated MscL channels. This method consists of lipid-encased aqueous droplets anchored to the tips of two opposing (coaxially positioned) borosilicate glass micropipettes. When droplets are brought into contact, a lipid bilayer interface is formed. This technique offers control over the chemical composition and the size of each droplet, as well as the dimensions of the bilayer interface. Having one of the micropipettes attached to a harmonic piezoelectric actuator provides the ability to deliver a desired oscillatory stimulus. Through analysis of the shapes of the droplets during deformation, the tension created at the interface can be estimated. Using this technique, the first activity of MscL channels in a DIB system is reported. Besides MS channels, activities of other types of channels can be studied using this method, proving the multi-functionality of this platform. The method presented here enables the measurement of fundamental membrane properties, provides a greater control over the formation of symmetric and asymmetric membranes, and is an alternative way to stimulate and study mechanosensitive channels.

Original languageEnglish (US)
JournalJournal of Visualized Experiments
Volume2015
Issue number105
DOIs
StatePublished - Nov 19 2015

Fingerprint

Ion Channels
Ions
Cell Membrane Permeability
Artificial Membranes
Equipment and Supplies
Membranes
Lipid Bilayers
Patch-Clamp Techniques
Transducers
Glass
Shock
Lipid bilayers
Borosilicate glass
Bacteria
Lipids
Piezoelectric actuators
Clamping devices
Cell membranes
Contacts (fluid mechanics)
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Najem, Joseph S. ; Dunlap, Myles D. ; Yasmann, Anthony ; Freeman, Eric C. ; Grant, John W. ; Sukharev, Sergei ; Leo, Donald J. / Multifunctional, micropipette-based method for incorporation and stimulation of bacterial mechanosensitive ion channels in droplet interface bilayers. In: Journal of Visualized Experiments. 2015 ; Vol. 2015, No. 105.
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Multifunctional, micropipette-based method for incorporation and stimulation of bacterial mechanosensitive ion channels in droplet interface bilayers. / Najem, Joseph S.; Dunlap, Myles D.; Yasmann, Anthony; Freeman, Eric C.; Grant, John W.; Sukharev, Sergei; Leo, Donald J.

In: Journal of Visualized Experiments, Vol. 2015, No. 105, 19.11.2015.

Research output: Contribution to journalArticle

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AU - Najem, Joseph S.

AU - Dunlap, Myles D.

AU - Yasmann, Anthony

AU - Freeman, Eric C.

AU - Grant, John W.

AU - Sukharev, Sergei

AU - Leo, Donald J.

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