Multilocus sequence typing (MLST) of leptospiral strains isolated from two geographic locations of Tamil Nadu, India

Murugesan Kanagavel, Alphonse Asirvatham Princy Margreat, Manivel Arunkumar, Shanmugarajan Gnanasekaran Prabhakaran, Shanmughapriya Santhanam, Kalimuthusamy Natarajaseenivasan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Here the rodent carrier status for the transmission of human leptospirosis in Tiruchirappalli, district, Tamil Nadu, India was assessed. The predominantly circulating leptospiral STs were recognized by multilocus sequence typing (MLST). A total of 113 rodents were trapped from different provinces of the Tiruchirappalli district. The most prevalent rodent was Bandicota bengalensis (37.2%), and of the total, 52.2% (n = 59) rodents were found to be positive for leptospiral 16S rRNA. These results were validated with a leptospiral culture positivity of 45.8% (n = 27). Three isolates from Chennai (2 rodents and 1 human) and 1 human isolate from Tiruchirappalli were included to understand the spatial variations and to track the source of human leptospirosis. The serogroup, serovar, and species level identification of all 31 isolates identified 28 to be Leptospira borgpetersenii serovar Javanica and three as Leptospira interrogans serovar Autumnalis. MLST analysis defined all isolates to the existing ST profiles (ST145 and ST27) with the exception of 6 L. borgpetersenii (ST DR) isolates that showed variations in the sucA and pfkB loci. The DR ST was locally confined to Chatram province of Tiruchirappalli suggesting an epidemiological link. The predominant STs, ST145 and ST-DR form a group, indicating the presence of original strain that subsequently diverged evolutionarily into two STs. The variations between L. borgpetersenii in sucA and pfkB loci may be an indication that evolutionary changes transpired in Tiruchirappalli.

Original languageEnglish (US)
Pages (from-to)123-128
Number of pages6
JournalInfection, Genetics and Evolution
Volume37
DOIs
StatePublished - Jan 1 2016

Fingerprint

Multilocus Sequence Typing
Geographic Locations
Leptospira borgpetersenii
rodent
India
Rodentia
rodents
serotypes
Leptospirosis
leptospirosis
Leptospira interrogans serovar autumnalis
Bandicota bengalensis
Murinae
carrier state
Leptospira
Leptospira interrogans
loci
spatial variation
multilocus sequence typing
ribosomal RNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Kanagavel, Murugesan ; Princy Margreat, Alphonse Asirvatham ; Arunkumar, Manivel ; Prabhakaran, Shanmugarajan Gnanasekaran ; Santhanam, Shanmughapriya ; Natarajaseenivasan, Kalimuthusamy. / Multilocus sequence typing (MLST) of leptospiral strains isolated from two geographic locations of Tamil Nadu, India. In: Infection, Genetics and Evolution. 2016 ; Vol. 37. pp. 123-128.
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Multilocus sequence typing (MLST) of leptospiral strains isolated from two geographic locations of Tamil Nadu, India. / Kanagavel, Murugesan; Princy Margreat, Alphonse Asirvatham; Arunkumar, Manivel; Prabhakaran, Shanmugarajan Gnanasekaran; Santhanam, Shanmughapriya; Natarajaseenivasan, Kalimuthusamy.

In: Infection, Genetics and Evolution, Vol. 37, 01.01.2016, p. 123-128.

Research output: Contribution to journalArticle

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AU - Princy Margreat, Alphonse Asirvatham

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AU - Prabhakaran, Shanmugarajan Gnanasekaran

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