Multiple, immunoidentical forms of phenobarbital-induced rat liver cytochromes P-450 are encoded by different mRNAs

F. G. Walz, G. P. Vlasuk, Curtis John Omiecinski, E. Bresnick, P. E. Thomas, D. E. Ryan, W. Levin

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Abstract

It was shown previously that four immunochemically identified forms of phenobarbital-induced hepatic cytochromic P-450 exist in unique combinations which characterize different strains and colonies of rats. One colony of Long-Evans rats exhibited only cytochromes P-450b(LE) and P-450e among these immunorelated enzymes; whereas, one colony of Holtzman rats was characterized by cytochromes P-450b(H) and P-450e. After phenobarbital treatment, hepatic poly(A)+-mRNA was isolated from these groups of rats and translated in vitro. The 35S-labeled products were immunoisolated with antibody to cytochrome P-450b(LE) and analyzed by two-dimensional gel electrophoresis. The results indicated that the products synthesized in vitro correspond exactly to the two particular forms of the enzyme that characterize liver microsomes from each of these groups of rats. It is concluded that different structural genes encode these immunorelated forms of cytochrome P-450 and that significant post-translational processing of their polypeptide products does not occur in vivo.

Original languageEnglish (US)
Pages (from-to)4023-4026
Number of pages4
JournalJournal of Biological Chemistry
Volume257
Issue number8
StatePublished - Nov 29 1982

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Cytochromes
Phenobarbital
Liver
Cytochrome P-450 Enzyme System
Rats
Messenger RNA
Long Evans Rats
Electrophoresis, Gel, Two-Dimensional
Liver Microsomes
Enzymes
Sprague Dawley Rats
Peptides
Electrophoresis
Antibodies
Genes
Gels
Processing
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Walz, F. G., Vlasuk, G. P., Omiecinski, C. J., Bresnick, E., Thomas, P. E., Ryan, D. E., & Levin, W. (1982). Multiple, immunoidentical forms of phenobarbital-induced rat liver cytochromes P-450 are encoded by different mRNAs. Journal of Biological Chemistry, 257(8), 4023-4026.
Walz, F. G. ; Vlasuk, G. P. ; Omiecinski, Curtis John ; Bresnick, E. ; Thomas, P. E. ; Ryan, D. E. ; Levin, W. / Multiple, immunoidentical forms of phenobarbital-induced rat liver cytochromes P-450 are encoded by different mRNAs. In: Journal of Biological Chemistry. 1982 ; Vol. 257, No. 8. pp. 4023-4026.
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Walz, FG, Vlasuk, GP, Omiecinski, CJ, Bresnick, E, Thomas, PE, Ryan, DE & Levin, W 1982, 'Multiple, immunoidentical forms of phenobarbital-induced rat liver cytochromes P-450 are encoded by different mRNAs', Journal of Biological Chemistry, vol. 257, no. 8, pp. 4023-4026.

Multiple, immunoidentical forms of phenobarbital-induced rat liver cytochromes P-450 are encoded by different mRNAs. / Walz, F. G.; Vlasuk, G. P.; Omiecinski, Curtis John; Bresnick, E.; Thomas, P. E.; Ryan, D. E.; Levin, W.

In: Journal of Biological Chemistry, Vol. 257, No. 8, 29.11.1982, p. 4023-4026.

Research output: Contribution to journalArticle

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N2 - It was shown previously that four immunochemically identified forms of phenobarbital-induced hepatic cytochromic P-450 exist in unique combinations which characterize different strains and colonies of rats. One colony of Long-Evans rats exhibited only cytochromes P-450b(LE) and P-450e among these immunorelated enzymes; whereas, one colony of Holtzman rats was characterized by cytochromes P-450b(H) and P-450e. After phenobarbital treatment, hepatic poly(A)+-mRNA was isolated from these groups of rats and translated in vitro. The 35S-labeled products were immunoisolated with antibody to cytochrome P-450b(LE) and analyzed by two-dimensional gel electrophoresis. The results indicated that the products synthesized in vitro correspond exactly to the two particular forms of the enzyme that characterize liver microsomes from each of these groups of rats. It is concluded that different structural genes encode these immunorelated forms of cytochrome P-450 and that significant post-translational processing of their polypeptide products does not occur in vivo.

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Walz FG, Vlasuk GP, Omiecinski CJ, Bresnick E, Thomas PE, Ryan DE et al. Multiple, immunoidentical forms of phenobarbital-induced rat liver cytochromes P-450 are encoded by different mRNAs. Journal of Biological Chemistry. 1982 Nov 29;257(8):4023-4026.