Multiple pathways for uptake of paraquat, methylglyoxal bis(guanylhydrazone), and polyamines

T. L. Byers, R. Kameji, D. E. Rannels, Anthony Pegg

Research output: Contribution to journalArticle

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Abstract

The uptake of polyamines, methylglyoxal bis(guanylhydrazone) MGBG), and paraquat [N,N-dimethyl-4,4'-bipyridylium] into control Chinese hamster ovary (CHO) cells and a mutant CHO cell line selected for resistance to the toxicity of MGBG was examined. In contrast to control CHO cells, the mutant cells had no detectable uptake of MGBG or any of the polyamines. There was no difference between the two cell lines in the uptake of α-aminoisobutyric acid (AIB), which indicates that there was no general change in membrane transport processes. The mutant cells were also found to be resistant to the toxicity of paraquat and to have a reduced capability to take up the herbicide. This finding confirms that the uptake of paraquat is necessary for the toxicity of this compound and that the paraquat is taken up by a transport system that also transports MGBG. Competition experiments showed that an excess of unlabeled paraquat inhibited uptake of MGBG and, to a lesser extent, uptake of putrescine and spermidine, but no inhibitory action on spermine uptake could be detected. Studies with type II cells isolated from rat lung also demonstrated uptake of paraquat and spermidine, but paraquat was only a weak inhibitor of spermidine uptake in this system. These results suggest that there may be multiple systems for the uptake of MGBG and polyamines and that paraquat is taken up by at least one but not all of these systems.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume252
Issue number6
StatePublished - Jan 1 1987

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Mitoguazone
Paraquat
Polyamines
Spermidine
Cricetulus
Ovary
Aminoisobutyric Acids
Cell Line
Putrescine
Spermine
Herbicides
Lung

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

Cite this

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title = "Multiple pathways for uptake of paraquat, methylglyoxal bis(guanylhydrazone), and polyamines",
abstract = "The uptake of polyamines, methylglyoxal bis(guanylhydrazone) MGBG), and paraquat [N,N-dimethyl-4,4'-bipyridylium] into control Chinese hamster ovary (CHO) cells and a mutant CHO cell line selected for resistance to the toxicity of MGBG was examined. In contrast to control CHO cells, the mutant cells had no detectable uptake of MGBG or any of the polyamines. There was no difference between the two cell lines in the uptake of α-aminoisobutyric acid (AIB), which indicates that there was no general change in membrane transport processes. The mutant cells were also found to be resistant to the toxicity of paraquat and to have a reduced capability to take up the herbicide. This finding confirms that the uptake of paraquat is necessary for the toxicity of this compound and that the paraquat is taken up by a transport system that also transports MGBG. Competition experiments showed that an excess of unlabeled paraquat inhibited uptake of MGBG and, to a lesser extent, uptake of putrescine and spermidine, but no inhibitory action on spermine uptake could be detected. Studies with type II cells isolated from rat lung also demonstrated uptake of paraquat and spermidine, but paraquat was only a weak inhibitor of spermidine uptake in this system. These results suggest that there may be multiple systems for the uptake of MGBG and polyamines and that paraquat is taken up by at least one but not all of these systems.",
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Multiple pathways for uptake of paraquat, methylglyoxal bis(guanylhydrazone), and polyamines. / Byers, T. L.; Kameji, R.; Rannels, D. E.; Pegg, Anthony.

In: American Journal of Physiology - Cell Physiology, Vol. 252, No. 6, 01.01.1987.

Research output: Contribution to journalArticle

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