Multiple red cell transfusions and alloimmunization: Experience with 6996 antibodies detected in a total of 159 262 patients from 1985 to 1993

G. A. Hoeltge, Ronald Domen, L. A. Rybicki, P. A. Schaffer

Research output: Contribution to journalArticlepeer-review

105 Scopus citations

Abstract

This retrospective study of red cell antibodies covered the period from 1985 to 1993. A three-cell antibody screen, 22% albumin enhancement, and a polyspecific antiglobulin reagent assay were performed. From a total of 159 262 patients in the data set, 6996 antibodies were detected among the sera of 4700 patients (2.9%). Four thousand two hundred thirty-five (60.5%) alloantibodies of potential clinical significance were found. These included anti-K1 (23.0%), -E (17.6%), -D (12.4%), -Lea (7.3%), -C (6.3%), -Fya (5.7%), and -c (4.4%). Cold agglutinins were found in 1119 positive antibody screens, 261 had warm autoantibodies, and 554 had high-titered, low-avidity antibodies. Three hundred seven were clinically insignificant (eg, Sd(a) and Bg(a)). Five hundred seventeen were too weak to identify. Most patients' sera demonstrated only one antibody (69.3%), but there was a strong linear correlation between the total number of recorded red cell transfusions and the number of antibodies found (r = .976; P < .0001). There was a higher percentage of females with antibodies than the percentage of females in the total study population (59.2% versus 43.8%, P < .0001). Two hundred nine of 554 (37.7%) high-titered, low-avidity antibodies and 349 (31.2%) of 1119 of the cold agglutinins accompanied or obscured clinically significant antibodies.

Original languageEnglish (US)
Pages (from-to)42-45
Number of pages4
JournalArchives of Pathology and Laboratory Medicine
Volume119
Issue number1
StatePublished - Jan 1 1995

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Fingerprint Dive into the research topics of 'Multiple red cell transfusions and alloimmunization: Experience with 6996 antibodies detected in a total of 159 262 patients from 1985 to 1993'. Together they form a unique fingerprint.

Cite this