Multiple roles of Epstein-Barr virus SM protein in lytic replication

Zhao Han, Elessa Marendy, Yong Dong Wang, Jing Yuan, Jeffery T. Sample, Sankar Swaminathan

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The effect of Epstein-Barr virus (EBV) SM protein on EBV gene expression was examined using a recombinant EBV strain with the SM gene deleted and DNA microarrays representing all known EBV coding regions. Induction of lytic EBV replication in the absence of SM led to expression of approximately 40% of EBV genes, but a block in expression of over 50% of EBV genes. Contrary to previous findings, several early genes were SM dependent, and lytic EBV DNA replication did not occur in the absence of SM. Notably, two genes essential for lytic EBV DNA replication, BSLF1 and BALF5, encoding EBV DNA primase and polymerase, respectively, were SM dependent. Lytic DNA replication was partially rescued by ectopic expression of EBV primase and polymerase, but virion production was not. Rescue of DNA replication only enhanced expression of a subset of late genes, consistent with a direct requirement for SM for late gene expression in addition to its contribution to DNA replication. Therefore, while SM is essential for most late gene expression, the proximate block to virion production by the EBV SM deletion strain is an inability to replicate linear DNA. The block to DNA replication combined with the direct effect of SM on late gene expression leads to a global deficiency of late gene expression. SM also inhibited BHRF1 expression during productive replication in comparison to that of cells induced into lytic replication in the absence of SM. Thus, SM plays a role in multiple steps of lytic cycle EBV gene expression and that it is transcript-specific in botli activation and repression functions.

Original languageEnglish (US)
Pages (from-to)4058-4069
Number of pages12
JournalJournal of virology
Volume81
Issue number8
DOIs
StatePublished - Apr 1 2007

Fingerprint

Human herpesvirus 4
Human Herpesvirus 4
DNA Replication
DNA replication
proteins
Gene Expression
gene expression
Virus Replication
DNA Primase
Genes
Virion
virion
genes
Human herpesvirus 4 BRLF1 protein
Essential Genes
Oligonucleotide Array Sequence Analysis
DNA
virus replication

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Han, Z., Marendy, E., Wang, Y. D., Yuan, J., Sample, J. T., & Swaminathan, S. (2007). Multiple roles of Epstein-Barr virus SM protein in lytic replication. Journal of virology, 81(8), 4058-4069. https://doi.org/10.1128/JVI.02665-06
Han, Zhao ; Marendy, Elessa ; Wang, Yong Dong ; Yuan, Jing ; Sample, Jeffery T. ; Swaminathan, Sankar. / Multiple roles of Epstein-Barr virus SM protein in lytic replication. In: Journal of virology. 2007 ; Vol. 81, No. 8. pp. 4058-4069.
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Han, Z, Marendy, E, Wang, YD, Yuan, J, Sample, JT & Swaminathan, S 2007, 'Multiple roles of Epstein-Barr virus SM protein in lytic replication', Journal of virology, vol. 81, no. 8, pp. 4058-4069. https://doi.org/10.1128/JVI.02665-06

Multiple roles of Epstein-Barr virus SM protein in lytic replication. / Han, Zhao; Marendy, Elessa; Wang, Yong Dong; Yuan, Jing; Sample, Jeffery T.; Swaminathan, Sankar.

In: Journal of virology, Vol. 81, No. 8, 01.04.2007, p. 4058-4069.

Research output: Contribution to journalArticle

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