Multiple tissue expression of AHR and arnt proteins in rats exposed to TCDD

V. M. Richardson, M. J. Santostefano, R. S. Pollenz, Gary H. Perdew, L. S. Birnbaum

Research output: Contribution to journalArticle

Abstract

This investigation examines the dose-response relationship between 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) treatment and aryl hydrocarbon receptor (AhR) and aryl hydrocarbon nuclear translocator (Amt) protein induction in multiple tissues of female Sprague-Dawley rats. Rats received a single oral dose of 0.0 (corn oil vehicle), 0.01, 0.1, 0.3, 1.0, 10.0 or 30.0 ug [3H]TCDD/kg body weight and were sacrificed three days after dosing. Hepatic, renal and pulmonary cytosolic extracts were prepared and subjected to western blotting analysis with AhR and Arm antibodies. The data suggests that in all tissues examined the cytosolic AhR and Arm proteins are expressed dose-independently. Nuclear extracts were prepared from the liver and also revealed that regulation of the AhR and Amt proteins is unaffected by dose. Further analysis of the functionality of the ligand:AhR:Arnt heterodimer complex by western blotting and enzyme induction techniques showed that both cytochrome P4501A1 (CYP1A1) protein and ethoxyresorufin O-deethylase (EROD) activity (a marker for CYP1A1 activity) were dose-dependently expressed in the liver, lungs and kidneys. This is the first study to show that regulation of the AhR and Arnt proteins in multiple tissues of female Sprague-Dawley rats is unaffected by acute exposure to TCDD.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996

Fingerprint

tetrachlorodibenzo-p-dioxin
Aryl Hydrocarbon Receptors
hydrocarbons
Rats
Tissue
rats
receptors
Proteins
proteins
Cytochromes
Liver
Sprague Dawley Rats
cytochromes
dosage
liver
Aryl Hydrocarbon Receptor Nuclear Translocator
Western Blotting
Western blotting
Kidney
lungs

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Richardson, V. M., Santostefano, M. J., Pollenz, R. S., Perdew, G. H., & Birnbaum, L. S. (1996). Multiple tissue expression of AHR and arnt proteins in rats exposed to TCDD. FASEB Journal, 10(3).
Richardson, V. M. ; Santostefano, M. J. ; Pollenz, R. S. ; Perdew, Gary H. ; Birnbaum, L. S. / Multiple tissue expression of AHR and arnt proteins in rats exposed to TCDD. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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Richardson, VM, Santostefano, MJ, Pollenz, RS, Perdew, GH & Birnbaum, LS 1996, 'Multiple tissue expression of AHR and arnt proteins in rats exposed to TCDD', FASEB Journal, vol. 10, no. 3.

Multiple tissue expression of AHR and arnt proteins in rats exposed to TCDD. / Richardson, V. M.; Santostefano, M. J.; Pollenz, R. S.; Perdew, Gary H.; Birnbaum, L. S.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

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AU - Richardson, V. M.

AU - Santostefano, M. J.

AU - Pollenz, R. S.

AU - Perdew, Gary H.

AU - Birnbaum, L. S.

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Richardson VM, Santostefano MJ, Pollenz RS, Perdew GH, Birnbaum LS. Multiple tissue expression of AHR and arnt proteins in rats exposed to TCDD. FASEB Journal. 1996;10(3).