Multiplex PCR assay simplifies serotyping and sequence typing of Listeria monocytogenes associated with human outbreaks

Wei Zhang, Stephen J. Knabel

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Listeria monocytogenes serotypes 1/2a and 4b are responsible for the majority of cases of human listeriosis worldwide. In this study, a multiplex PCR assay was developed to allow rapid identification and easily interpretable differentiation of serotypes 1/2a and 4b from other serotypes of L. monocytogenes by simultaneously targeting two virulence genes (inlB and inlC) and two serotype-specific genes (ORF2372 and lmo0171). A subsequent gel extraction and sequence typing analysis of the highly polymorphic intragenic regions in inlB and inlC simplified a previously developed multi-virulence-locus sequence typing scheme and provided discriminatory power for subtyping L. monocytogenes similar to pulsed-field gel electrophoresis analysis.

Original languageEnglish (US)
Pages (from-to)1907-1910
Number of pages4
JournalJournal of Food Protection
Volume68
Issue number9
DOIs
StatePublished - Sep 2005

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology

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