Multiplex systems for the amplification of short tandem repeat loci: Evaluation of laser fluorescence detection

Marie D. Ricciardone, Ann M. Lins, James W. Schumm, Mitchell Mark Holland

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Short tandem repeat (STR) loci are ideal markers for personal identification and for genomic mapping. Two fluorescent multiplex systems, each designed for simultaneous PCR amplification of four polymorphic STR loci (HUMCSF1PO, HUMTPOX, HUMTH01 and HUMVWFA31, and HUMF13A01, HUMFESFPS, HUMBFXIII and HUMLIPOL), were evaluated on three laser fluorescence detection instruments. Concordant DNA typing results were obtained with all three detection methods. These fluorescent multiplex STR systems offer an accurate, reproducible and versatile method of DNA profiling that is well-suited for forensic identity testing and other genetic analyses.

Original languageEnglish (US)
Pages (from-to)742-747
Number of pages6
JournalBioTechniques
Volume23
Issue number4
StatePublished - Oct 1 1997

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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