Multiscale stress–strain characterization of onion outer epidermal tissue in wet and dry states

Keekyoung Kim, Hojae Yi, M. Shafayet Zamil, Md Amanul Haque, Virendra Puri

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

• Premise of the study: Quantitative measurements of water’s effects on the tension response of plant tissue will assist in understanding the regulatory mechanism underlying expansive growth. Such measurements should be multiscale in nature to account for plants’ hierarchical structure.

• Methods: Outer onion epidermal tissues were cut and bonded to uniaxial displacement-controlled mechanical loading devices to apply and measure the force on the sample. Fluorescent polystyrene beads (500 nm in diameter) were dispersed on the sample surface under various levels of tensile load conditions to obtain displacement maps with a confocal fluorescent microscope. The resulting strain was measured using a digital image correlation technique by tracking individual bead displacements. The applied forces were obtained by measuring the displacement of the calibrated force-sensing device. Tissue- and cell-scale mechanical properties were quantified by calculating the applied stress and the corresponding global and local strains.

• Key results: The Young’s modulus values of individual cell walls of dehydrated and rehydrated samples were 3.0 ± 1.0 GPa and 0.4 ± 0.2 GPa, respectively, and are different from the Young’s modulus values of the global tissue-scale dehydrated and rehydrated samples, which were 1.9 ± 0.3 GPa and 0.08 ± 0.02 GPa, respectively. Poisson’s ratio increased more than 3-fold due to hydration.

• Conclusion: The results on global, cell-to-cell, and point-to-point mechanical property variations suggest the importance of the mechanical contribution of extracellular features including the middle lamella, cell shape, and dimension. This study shows that a multiscale investigation is essential for fundamental insights into the hierarchical deformation of biological systems.

Original languageEnglish (US)
Pages (from-to)12-20
Number of pages9
JournalAmerican journal of botany
Volume102
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

Onions
onions
Elastic Modulus
Young modulus
modulus of elasticity
mechanical properties
mechanical property
cells
Plant Structures
sampling
Equipment and Supplies
Cell Shape
Polystyrenes
Poisson ratio
polystyrenes
digital images
digital image
hydration
microscopes
Cell Wall

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

Cite this

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abstract = "• Premise of the study: Quantitative measurements of water’s effects on the tension response of plant tissue will assist in understanding the regulatory mechanism underlying expansive growth. Such measurements should be multiscale in nature to account for plants’ hierarchical structure.• Methods: Outer onion epidermal tissues were cut and bonded to uniaxial displacement-controlled mechanical loading devices to apply and measure the force on the sample. Fluorescent polystyrene beads (500 nm in diameter) were dispersed on the sample surface under various levels of tensile load conditions to obtain displacement maps with a confocal fluorescent microscope. The resulting strain was measured using a digital image correlation technique by tracking individual bead displacements. The applied forces were obtained by measuring the displacement of the calibrated force-sensing device. Tissue- and cell-scale mechanical properties were quantified by calculating the applied stress and the corresponding global and local strains.• Key results: The Young’s modulus values of individual cell walls of dehydrated and rehydrated samples were 3.0 ± 1.0 GPa and 0.4 ± 0.2 GPa, respectively, and are different from the Young’s modulus values of the global tissue-scale dehydrated and rehydrated samples, which were 1.9 ± 0.3 GPa and 0.08 ± 0.02 GPa, respectively. Poisson’s ratio increased more than 3-fold due to hydration.• Conclusion: The results on global, cell-to-cell, and point-to-point mechanical property variations suggest the importance of the mechanical contribution of extracellular features including the middle lamella, cell shape, and dimension. This study shows that a multiscale investigation is essential for fundamental insights into the hierarchical deformation of biological systems.",
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Multiscale stress–strain characterization of onion outer epidermal tissue in wet and dry states. / Kim, Keekyoung; Yi, Hojae; Shafayet Zamil, M.; Haque, Md Amanul; Puri, Virendra.

In: American journal of botany, Vol. 102, No. 1, 01.01.2015, p. 12-20.

Research output: Contribution to journalArticle

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