Multivalent dendrimeric compounds containing carbohydrates expressed on immune cells inhibit infection by primary isolates of HIV-1

Andrew Rosa Borges, Lindsay Wieczorek, Benitra Johnson, Alan J. Benesi, Bruce K. Brown, Richard D. Kensinger, Fred C. Krebs, Brian Wigdahl, Robert Blumenthal, Anu Puri, Francine E. McCutchan, Deborah L. Birx, Victoria R. Polonis, Cara Lynne Schengrund

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Specific glycosphingolipids (GSL), found on the surface of target immune cells, are recognized as alternate cell surface receptors by the human immunodeficiency virus type 1 (HIV-1) external envelope glycoprotein. In this study, the globotriose and 3'-sialyllactose carbohydrate head groups found on two GSL were covalently attached to a dendrimer core to produce two types of unique multivalent carbohydrates (MVC). These MVC inhibited HIV-1 infection of T cell lines and primary peripheral blood mononuclear cells (PBMC) by T cell line-adapted viruses or primary isolates, with IC50s ranging from 0.1 to 7.4μg/ml. Inhibition of Env-mediated membrane fusion by MVC was also observed using a dye-transfer assay. These carbohydrate compounds warrant further investigation as a potential new class of HIV-1 entry inhibitors. The data presented also shed light on the role of carbohydrate moieties in HIV-1 virus-host cell interactions.

Original languageEnglish (US)
Pages (from-to)80-88
Number of pages9
JournalVirology
Volume408
Issue number1
DOIs
StatePublished - Dec 5 2010

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HIV-1
Carbohydrates
Infection
Glycosphingolipids
Viruses
T-Lymphocytes
Dendrimers
Cell Line
Virus Internalization
Membrane Fusion
Cell Surface Receptors
Virus Diseases
Cell Communication
Blood Cells
Glycoproteins
Coloring Agents

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Rosa Borges, Andrew ; Wieczorek, Lindsay ; Johnson, Benitra ; Benesi, Alan J. ; Brown, Bruce K. ; Kensinger, Richard D. ; Krebs, Fred C. ; Wigdahl, Brian ; Blumenthal, Robert ; Puri, Anu ; McCutchan, Francine E. ; Birx, Deborah L. ; Polonis, Victoria R. ; Schengrund, Cara Lynne. / Multivalent dendrimeric compounds containing carbohydrates expressed on immune cells inhibit infection by primary isolates of HIV-1. In: Virology. 2010 ; Vol. 408, No. 1. pp. 80-88.
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abstract = "Specific glycosphingolipids (GSL), found on the surface of target immune cells, are recognized as alternate cell surface receptors by the human immunodeficiency virus type 1 (HIV-1) external envelope glycoprotein. In this study, the globotriose and 3'-sialyllactose carbohydrate head groups found on two GSL were covalently attached to a dendrimer core to produce two types of unique multivalent carbohydrates (MVC). These MVC inhibited HIV-1 infection of T cell lines and primary peripheral blood mononuclear cells (PBMC) by T cell line-adapted viruses or primary isolates, with IC50s ranging from 0.1 to 7.4μg/ml. Inhibition of Env-mediated membrane fusion by MVC was also observed using a dye-transfer assay. These carbohydrate compounds warrant further investigation as a potential new class of HIV-1 entry inhibitors. The data presented also shed light on the role of carbohydrate moieties in HIV-1 virus-host cell interactions.",
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Rosa Borges, A, Wieczorek, L, Johnson, B, Benesi, AJ, Brown, BK, Kensinger, RD, Krebs, FC, Wigdahl, B, Blumenthal, R, Puri, A, McCutchan, FE, Birx, DL, Polonis, VR & Schengrund, CL 2010, 'Multivalent dendrimeric compounds containing carbohydrates expressed on immune cells inhibit infection by primary isolates of HIV-1', Virology, vol. 408, no. 1, pp. 80-88. https://doi.org/10.1016/j.virol.2010.09.004

Multivalent dendrimeric compounds containing carbohydrates expressed on immune cells inhibit infection by primary isolates of HIV-1. / Rosa Borges, Andrew; Wieczorek, Lindsay; Johnson, Benitra; Benesi, Alan J.; Brown, Bruce K.; Kensinger, Richard D.; Krebs, Fred C.; Wigdahl, Brian; Blumenthal, Robert; Puri, Anu; McCutchan, Francine E.; Birx, Deborah L.; Polonis, Victoria R.; Schengrund, Cara Lynne.

In: Virology, Vol. 408, No. 1, 05.12.2010, p. 80-88.

Research output: Contribution to journalArticle

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AU - Rosa Borges, Andrew

AU - Wieczorek, Lindsay

AU - Johnson, Benitra

AU - Benesi, Alan J.

AU - Brown, Bruce K.

AU - Kensinger, Richard D.

AU - Krebs, Fred C.

AU - Wigdahl, Brian

AU - Blumenthal, Robert

AU - Puri, Anu

AU - McCutchan, Francine E.

AU - Birx, Deborah L.

AU - Polonis, Victoria R.

AU - Schengrund, Cara Lynne

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