Multivalent dendrimeric compounds containing carbohydrates expressed on immune cells inhibit infection by primary isolates of HIV-1

Andrew Rosa Borges, Lindsay Wieczorek, Benitra Johnson, Alan J. Benesi, Bruce K. Brown, Richard D. Kensinger, Fred C. Krebs, Brian Wigdahl, Robert Blumenthal, Anu Puri, Francine E. McCutchan, Deborah L. Birx, Victoria R. Polonis, Cara Lynne Schengrund

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Specific glycosphingolipids (GSL), found on the surface of target immune cells, are recognized as alternate cell surface receptors by the human immunodeficiency virus type 1 (HIV-1) external envelope glycoprotein. In this study, the globotriose and 3'-sialyllactose carbohydrate head groups found on two GSL were covalently attached to a dendrimer core to produce two types of unique multivalent carbohydrates (MVC). These MVC inhibited HIV-1 infection of T cell lines and primary peripheral blood mononuclear cells (PBMC) by T cell line-adapted viruses or primary isolates, with IC50s ranging from 0.1 to 7.4μg/ml. Inhibition of Env-mediated membrane fusion by MVC was also observed using a dye-transfer assay. These carbohydrate compounds warrant further investigation as a potential new class of HIV-1 entry inhibitors. The data presented also shed light on the role of carbohydrate moieties in HIV-1 virus-host cell interactions.

Original languageEnglish (US)
Pages (from-to)80-88
Number of pages9
JournalVirology
Volume408
Issue number1
DOIs
StatePublished - Dec 5 2010

All Science Journal Classification (ASJC) codes

  • Virology

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    Rosa Borges, A., Wieczorek, L., Johnson, B., Benesi, A. J., Brown, B. K., Kensinger, R. D., Krebs, F. C., Wigdahl, B., Blumenthal, R., Puri, A., McCutchan, F. E., Birx, D. L., Polonis, V. R., & Schengrund, C. L. (2010). Multivalent dendrimeric compounds containing carbohydrates expressed on immune cells inhibit infection by primary isolates of HIV-1. Virology, 408(1), 80-88. https://doi.org/10.1016/j.virol.2010.09.004