Multivariate statistical analyses demonstrate unique host immune responses to single and dual lentiviral infection

Sunando Roy, Jennie Lavine, Francesca Chiaromonte, Julie Terwee, Sue VandeWoude, Ottar Bjornstad, Mary Poss

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Feline immunodeficiency virus (FIV) and human immunodeficiency virus (HIV) are recently identified lentiviruses that cause progressive immune decline and ultimately death in infected cats and humans. It is of great interest to understand how to prevent immune system collapse caused by these lentiviruses. We recently described that disease caused by a virulent FIV strain in cats can be attenuated if animals are first infected with a feline immunodeficiency virus derived from a wild cougar. The detailed temporal tracking of cat immunological parameters in response to two viral infections resulted in high-dimensional datasets containing variables that exhibit strong co-variation. Initial analyses of these complex data using univariate statistical techniques did not account for interactions among immunological response variables and therefore potentially obscured significant effects between infection state and immunological parameters. Methodology and Principal Findings: Here, we apply a suite of multivariate statistical tools, including Principal Component Analysis, MANOVA and Linear Discriminant Analysis, to temporal immunological data resulting from FIV superinfection in domestic cats. We investigated the co-variation among immunological responses, the differences in immune parameters among four groups of five cats each (uninfected, single and dual infected animals), and the "immune profiles" that discriminate among them over the first four weeks following superinfection. Dual infected cats mount an immune response by 24 days post superinfection that is characterized by elevated levels of CD8 and CD25 cells and increased expression of IL4 and IFNc, and FAS. This profile discriminates dual infected cats from cats infected with FIV alone, which show high IL-10 and lower numbers of CD8 and CD25 cells. Conclusions: Multivariate statistical analyses demonstrate both the dynamic nature of the immune response to FIV single and dual infection and the development of a unique immunological profile in dual infected cats, which are protected from immune decline.

Original languageEnglish (US)
Article numbere7359
JournalPloS one
Volume4
Issue number10
DOIs
StatePublished - Oct 6 2009

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Viruses
Feline Immunodeficiency Virus
Feline immunodeficiency virus
Cats
Multivariate Analysis
immune response
cats
Infection
infection
Superinfection
Lentivirus
Animals
Puma
Immune system
Discriminant analysis
Puma concolor
Interleukin-4
Interleukin-10
Principal component analysis
Human immunodeficiency virus

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

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title = "Multivariate statistical analyses demonstrate unique host immune responses to single and dual lentiviral infection",
abstract = "Background: Feline immunodeficiency virus (FIV) and human immunodeficiency virus (HIV) are recently identified lentiviruses that cause progressive immune decline and ultimately death in infected cats and humans. It is of great interest to understand how to prevent immune system collapse caused by these lentiviruses. We recently described that disease caused by a virulent FIV strain in cats can be attenuated if animals are first infected with a feline immunodeficiency virus derived from a wild cougar. The detailed temporal tracking of cat immunological parameters in response to two viral infections resulted in high-dimensional datasets containing variables that exhibit strong co-variation. Initial analyses of these complex data using univariate statistical techniques did not account for interactions among immunological response variables and therefore potentially obscured significant effects between infection state and immunological parameters. Methodology and Principal Findings: Here, we apply a suite of multivariate statistical tools, including Principal Component Analysis, MANOVA and Linear Discriminant Analysis, to temporal immunological data resulting from FIV superinfection in domestic cats. We investigated the co-variation among immunological responses, the differences in immune parameters among four groups of five cats each (uninfected, single and dual infected animals), and the {"}immune profiles{"} that discriminate among them over the first four weeks following superinfection. Dual infected cats mount an immune response by 24 days post superinfection that is characterized by elevated levels of CD8 and CD25 cells and increased expression of IL4 and IFNc, and FAS. This profile discriminates dual infected cats from cats infected with FIV alone, which show high IL-10 and lower numbers of CD8 and CD25 cells. Conclusions: Multivariate statistical analyses demonstrate both the dynamic nature of the immune response to FIV single and dual infection and the development of a unique immunological profile in dual infected cats, which are protected from immune decline.",
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Multivariate statistical analyses demonstrate unique host immune responses to single and dual lentiviral infection. / Roy, Sunando; Lavine, Jennie; Chiaromonte, Francesca; Terwee, Julie; VandeWoude, Sue; Bjornstad, Ottar; Poss, Mary.

In: PloS one, Vol. 4, No. 10, e7359, 06.10.2009.

Research output: Contribution to journalArticle

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AU - Roy, Sunando

AU - Lavine, Jennie

AU - Chiaromonte, Francesca

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AU - VandeWoude, Sue

AU - Bjornstad, Ottar

AU - Poss, Mary

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