Murine aerosol challenge model of anthrax

Crystal L. Loving, Mary J. Kennett, Gloria M. Lee, Vanessa K. Grippe, Tod J. Merkel

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The availability of relevant and useful animal models is critical for progress in the development of effective vaccines and therapeutics. The infection of rabbits and non-human primates with fully virulent Bacillus anthracis spores provides two excellent models of anthrax disease. However, the high cost of procuring and housing these animals and the specialized facilities required to deliver fully virulent spores limit their practical use in early stages of product development. Conversely, the small size and low cost associated with using mice makes this animal model more practical for conducting experiments in which large numbers of animals are required. In addition, the availability of knockout strains and well-characterized immunological reagents makes it possible to perform studies in mice that cannot be performed easily in other species. Although we, along with others, have used the mouse aerosol challenge model to examine the outcome of B. anthracis infection, a detailed characterization of the disease is lacking. The current study utilizes a murine aerosol challenge model to investigate disease progression, innate cytokine responses, and histological changes during the course of anthrax after challenge with aerosolized spores. Our results show that anthrax disease progression in a complement-deficient mouse after challenge with aerosolized Sterne spores is similar to that described for other species, including rabbits and non-human primates, challenged with fully virulent B. anthracis. Thus, the murine aerosol challenge model is both useful and relevant and provides a means to further investigate the host response and mechanisms of B. anthracis pathogenesis.

Original languageEnglish (US)
Pages (from-to)2689-2698
Number of pages10
JournalInfection and Immunity
Volume75
Issue number6
DOIs
StatePublished - Jun 1 2007

Fingerprint

Bacillus anthracis
Anthrax
Spores
Aerosols
Primates
Disease Progression
Animal Housing
Animal Models
Rabbits
Costs and Cost Analysis
Infection
Vaccines
Cytokines
Therapeutics

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Loving, C. L., Kennett, M. J., Lee, G. M., Grippe, V. K., & Merkel, T. J. (2007). Murine aerosol challenge model of anthrax. Infection and Immunity, 75(6), 2689-2698. https://doi.org/10.1128/IAI.01875-06
Loving, Crystal L. ; Kennett, Mary J. ; Lee, Gloria M. ; Grippe, Vanessa K. ; Merkel, Tod J. / Murine aerosol challenge model of anthrax. In: Infection and Immunity. 2007 ; Vol. 75, No. 6. pp. 2689-2698.
@article{bedab19ec4d949729147b05d79a1f512,
title = "Murine aerosol challenge model of anthrax",
abstract = "The availability of relevant and useful animal models is critical for progress in the development of effective vaccines and therapeutics. The infection of rabbits and non-human primates with fully virulent Bacillus anthracis spores provides two excellent models of anthrax disease. However, the high cost of procuring and housing these animals and the specialized facilities required to deliver fully virulent spores limit their practical use in early stages of product development. Conversely, the small size and low cost associated with using mice makes this animal model more practical for conducting experiments in which large numbers of animals are required. In addition, the availability of knockout strains and well-characterized immunological reagents makes it possible to perform studies in mice that cannot be performed easily in other species. Although we, along with others, have used the mouse aerosol challenge model to examine the outcome of B. anthracis infection, a detailed characterization of the disease is lacking. The current study utilizes a murine aerosol challenge model to investigate disease progression, innate cytokine responses, and histological changes during the course of anthrax after challenge with aerosolized spores. Our results show that anthrax disease progression in a complement-deficient mouse after challenge with aerosolized Sterne spores is similar to that described for other species, including rabbits and non-human primates, challenged with fully virulent B. anthracis. Thus, the murine aerosol challenge model is both useful and relevant and provides a means to further investigate the host response and mechanisms of B. anthracis pathogenesis.",
author = "Loving, {Crystal L.} and Kennett, {Mary J.} and Lee, {Gloria M.} and Grippe, {Vanessa K.} and Merkel, {Tod J.}",
year = "2007",
month = "6",
day = "1",
doi = "10.1128/IAI.01875-06",
language = "English (US)",
volume = "75",
pages = "2689--2698",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "6",

}

Loving, CL, Kennett, MJ, Lee, GM, Grippe, VK & Merkel, TJ 2007, 'Murine aerosol challenge model of anthrax', Infection and Immunity, vol. 75, no. 6, pp. 2689-2698. https://doi.org/10.1128/IAI.01875-06

Murine aerosol challenge model of anthrax. / Loving, Crystal L.; Kennett, Mary J.; Lee, Gloria M.; Grippe, Vanessa K.; Merkel, Tod J.

In: Infection and Immunity, Vol. 75, No. 6, 01.06.2007, p. 2689-2698.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Murine aerosol challenge model of anthrax

AU - Loving, Crystal L.

AU - Kennett, Mary J.

AU - Lee, Gloria M.

AU - Grippe, Vanessa K.

AU - Merkel, Tod J.

PY - 2007/6/1

Y1 - 2007/6/1

N2 - The availability of relevant and useful animal models is critical for progress in the development of effective vaccines and therapeutics. The infection of rabbits and non-human primates with fully virulent Bacillus anthracis spores provides two excellent models of anthrax disease. However, the high cost of procuring and housing these animals and the specialized facilities required to deliver fully virulent spores limit their practical use in early stages of product development. Conversely, the small size and low cost associated with using mice makes this animal model more practical for conducting experiments in which large numbers of animals are required. In addition, the availability of knockout strains and well-characterized immunological reagents makes it possible to perform studies in mice that cannot be performed easily in other species. Although we, along with others, have used the mouse aerosol challenge model to examine the outcome of B. anthracis infection, a detailed characterization of the disease is lacking. The current study utilizes a murine aerosol challenge model to investigate disease progression, innate cytokine responses, and histological changes during the course of anthrax after challenge with aerosolized spores. Our results show that anthrax disease progression in a complement-deficient mouse after challenge with aerosolized Sterne spores is similar to that described for other species, including rabbits and non-human primates, challenged with fully virulent B. anthracis. Thus, the murine aerosol challenge model is both useful and relevant and provides a means to further investigate the host response and mechanisms of B. anthracis pathogenesis.

AB - The availability of relevant and useful animal models is critical for progress in the development of effective vaccines and therapeutics. The infection of rabbits and non-human primates with fully virulent Bacillus anthracis spores provides two excellent models of anthrax disease. However, the high cost of procuring and housing these animals and the specialized facilities required to deliver fully virulent spores limit their practical use in early stages of product development. Conversely, the small size and low cost associated with using mice makes this animal model more practical for conducting experiments in which large numbers of animals are required. In addition, the availability of knockout strains and well-characterized immunological reagents makes it possible to perform studies in mice that cannot be performed easily in other species. Although we, along with others, have used the mouse aerosol challenge model to examine the outcome of B. anthracis infection, a detailed characterization of the disease is lacking. The current study utilizes a murine aerosol challenge model to investigate disease progression, innate cytokine responses, and histological changes during the course of anthrax after challenge with aerosolized spores. Our results show that anthrax disease progression in a complement-deficient mouse after challenge with aerosolized Sterne spores is similar to that described for other species, including rabbits and non-human primates, challenged with fully virulent B. anthracis. Thus, the murine aerosol challenge model is both useful and relevant and provides a means to further investigate the host response and mechanisms of B. anthracis pathogenesis.

UR - http://www.scopus.com/inward/record.url?scp=34249880799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249880799&partnerID=8YFLogxK

U2 - 10.1128/IAI.01875-06

DO - 10.1128/IAI.01875-06

M3 - Article

VL - 75

SP - 2689

EP - 2698

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 6

ER -