Murine gammaherpesvirus M2 gene is latency-associated and its protein a target for CD8+ T lymphocytes

S. Mazher Husain, Edward J. Usherwood, Heather Dyson, Christopher Coleclough, Michael A. Coppola, David L. Woodland, Marcia A. Blackman, James P. Stewart, Jeffery Sample

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Murine gammaherpesvirus 68 (MHV-68) infection of mice is a potential model with which to address fundamental aspects of the pathobiology and host control of gammaherpesvirus latency. Control of MHV-68 infection, like that of Epstein-Barr virus, is strongly dependent on the cellular immune system. However, the molecular biology of MHV-68 latency is largely undefined. A screen of the MHV-68 genome for potential latency-associated mRNAs revealed that the region encompassing and flanking the genomic terminal repeats is transcriptionally active in the latently infected murine B-cell tumor line S11. Transcription of one MHV-68 gene, that encoding the hypothetical M2 protein, was detected in virtually all latently infected S11 cells and in splenocytes of latently infected mice, but not in lyrically infected fibroblasts. Furthermore, an epitope was identified in the predicted M2 protein that is recognized by CD8+ T cells from infected mice and a cytotoxic T lymphocyte line that recognizes this epitope killed S11 cells, indicating that the M2 protein is expressed during latent infection and is a target for the host cytotoxic T lymphocyte response. This work therefore provides essential information for modeling MHV-68 latency and strategies of immunotherapy against gammaherpesvirus-related diseases in a highly tractable animal model.

Original languageEnglish (US)
Pages (from-to)7508-7513
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number13
DOIs
StatePublished - Jun 22 1999

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Cytotoxic T-Lymphocytes
T-Lymphocytes
Epitopes
Genes
Proteins
Epstein-Barr Virus Infections
Terminal Repeat Sequences
Infection
Immunotherapy
Molecular Biology
Immune System
B-Lymphocytes
Animal Models
Fibroblasts
Genome
Cell Line
Messenger RNA
Neoplasms

All Science Journal Classification (ASJC) codes

  • General

Cite this

Husain, S. Mazher ; Usherwood, Edward J. ; Dyson, Heather ; Coleclough, Christopher ; Coppola, Michael A. ; Woodland, David L. ; Blackman, Marcia A. ; Stewart, James P. ; Sample, Jeffery. / Murine gammaherpesvirus M2 gene is latency-associated and its protein a target for CD8+ T lymphocytes. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 13. pp. 7508-7513.
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Murine gammaherpesvirus M2 gene is latency-associated and its protein a target for CD8+ T lymphocytes. / Husain, S. Mazher; Usherwood, Edward J.; Dyson, Heather; Coleclough, Christopher; Coppola, Michael A.; Woodland, David L.; Blackman, Marcia A.; Stewart, James P.; Sample, Jeffery.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 13, 22.06.1999, p. 7508-7513.

Research output: Contribution to journalArticle

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T1 - Murine gammaherpesvirus M2 gene is latency-associated and its protein a target for CD8+ T lymphocytes

AU - Husain, S. Mazher

AU - Usherwood, Edward J.

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AU - Coppola, Michael A.

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AU - Sample, Jeffery

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