Muscarine receptor activation in the substantia gelatinosa of the spinal trigeminal nucleus of the guinea pig

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Abstract

1. Intracellular recordings were made from slices of guinea pig spinal trigeminal nucleus pars caudalis (SG). 2. Muscarine [0.3-30 μM: half maximally effective concentration (EC50) = 2.9 μM) hyperpolarized 61% of SG neurons. The effect was mimicked by carbachol (0.3-30 μM; EC50 = 3.9 μM) and antagonized by pirenzepine (1 μM). Thirty-four percent of the neurons were depolarized by muscarine and carbachol (1-30 μM; EC50 = 5.7 μM), and the effect was antagonized by pirenzepine (100 nM). 3. In ~80% of recordings, muscarine (10-30 μM) evoked repetitive spontaneous inhibitory postsynaptic potentials (IPSPs) that were sensitive to bicuculline (10 μM). 4. Muscarine (1-30 μM; EC50 = 3 μM) decreased the amplitude of the majority of evoked excitatory postsynaptic potentials (EPSPs), and the effect was mimicked by carbachol and antagonized by pirenzepine (100 nM). 5. These results indicate that there are at least three mechanisms by which muscarine inhibits SG neurons: 1) hyperpolarization through activation of non-M1 receptors; 2) activation of γ-amino-butyric acid-containing interneurons that mediate IPSPs in a subset of neurons; and 3) a decrease in evoked EPSP amplitude. Muscarine can also activate SG neurons via interaction with an M1-type receptor.

Original languageEnglish (US)
Pages (from-to)3817-3822
Number of pages6
JournalJournal of neurophysiology
Volume76
Issue number6
DOIs
Publication statusPublished - Dec 1996

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All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

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