Muscarinic acetylcholine receptor subtypes: Localization and structure/function

Mark R. Brann, John Ellis, Hans Jørgensen, David Hill-Eubanks, S. V.Penelope Jones

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Muscarinic receptors are purified to apparent homogeneity from the both porcine brain and heart. Sufficient peptide sequence is obtained from these preparations to allow the cloning of the ml and m2 muscarinic receptor subtypes from these tissues. Using homology cloning, the human and rat forms of these receptors as well as three additional subtypes (m3-m5) are identified. Based on the sequence of the five cloned muscarinic receptor subtypes (ml-m5), subtype selective antibody and cDNA probes have been prepared. Use of these probes has demonstrated that each of the five subtypes has a markedly distinct distribution within the brain and among peripheral tissues. The distributions of these subtypes and their potential physiological roles are discussed. By the use of molecular genetic manipulation of cloned muscarinic receptor cDNAs, the regions of muscarinic receptors that specify G-protein coupling and ligand binding have been defined in several recent studies. Overall, these studies have shown that amino acids within the third cytoplasmic loop of the receptors define their selectivities for different G-proteins and that multiple discontinuous epitopes contribute to their selectivity for different ligands. In this chapter, the residues that contribute to ligand binding and G-protein coupling are described, as well as the implied structures of these functional domains.

Original languageEnglish (US)
Pages (from-to)121-127
Number of pages7
JournalProgress in Brain Research
Volume98
Issue numberC
DOIs
StatePublished - Jan 1 1993

Fingerprint

Muscarinic Receptors
GTP-Binding Proteins
Ligands
Organism Cloning
Complementary DNA
Muscarinic M2 Receptors
Brain
Cytoplasmic and Nuclear Receptors
Epitopes
Molecular Biology
Carrier Proteins
Swine
Amino Acids
Peptides
Antibodies

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Brann, Mark R. ; Ellis, John ; Jørgensen, Hans ; Hill-Eubanks, David ; Jones, S. V.Penelope. / Muscarinic acetylcholine receptor subtypes : Localization and structure/function. In: Progress in Brain Research. 1993 ; Vol. 98, No. C. pp. 121-127.
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Muscarinic acetylcholine receptor subtypes : Localization and structure/function. / Brann, Mark R.; Ellis, John; Jørgensen, Hans; Hill-Eubanks, David; Jones, S. V.Penelope.

In: Progress in Brain Research, Vol. 98, No. C, 01.01.1993, p. 121-127.

Research output: Contribution to journalArticle

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