Muscarinic M1 Receptors Modulate Working Memory Performance and Activity via KCNQ Potassium Channels in the Primate Prefrontal Cortex

Veronica C. Galvin, Sheng Tao Yang, Constantinos D. Paspalas, Yang Yang, Lu E. Jin, Dibyadeep Datta, Yury M. Morozov, Taber C. Lightbourne, Adam S. Lowet, Pasko Rakic, Amy F.T. Arnsten, Min Wang

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Working memory relies on the dorsolateral prefrontal cortex (dlPFC), where microcircuits of pyramidal neurons enable persistent firing in the absence of sensory input, maintaining information through recurrent excitation. This activity relies on acetylcholine, although the molecular mechanisms for this dependence are not thoroughly understood. This study investigated the role of muscarinic M1 receptors (M1Rs) in the dlPFC using iontophoresis coupled with single-unit recordings from aging monkeys with naturally occurring cholinergic depletion. We found that M1R stimulation produced an inverted-U dose response on cell firing and behavioral performance when given systemically to aged monkeys. Immunoelectron microscopy localized KCNQ isoforms (Kv7.2, Kv7.3, and Kv7.5) on layer III dendrites and spines, similar to M1Rs. Iontophoretic manipulation of KCNQ channels altered cell firing and reversed the effects of M1R compounds, suggesting that KCNQ channels are one mechanism for M1R actions in the dlPFC. These results indicate that M1Rs may be an appropriate target to treat cognitive disorders with cholinergic alterations.

Original languageEnglish (US)
Pages (from-to)649-661.e4
JournalNeuron
Volume106
Issue number4
DOIs
StatePublished - May 20 2020

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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