Muscle oxygenation during dynamic plantar flexion exercise: combining BOLD MRI with traditional physiological measurements

Matthew D. Muller, Zhijun Li, Christopher T. Sica, J. Carter Luck, Zhaohui Gao, Cheryl A. Blaha, Aimee E. Cauffman, Amanda J. Ross, Nathan J.R. Winkler, Michael D. Herr, Kristen Brandt, Jianli Wang, David C. Gallagher, Prasanna Karunanayaka, Jeffrey Vesek, Urs A. Leuenberger, Qing X. Yang, Lawrence I. Sinoway

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Blood-oxygen-level-dependent magnetic resonance imaging (BOLD MRI) has the potential to quantify skeletal muscle oxygenation with high temporal and high spatial resolution. The purpose of this study was to characterize skeletal muscle BOLD responses during steady-state plantar flexion exercise (i.e., during the brief rest periods between muscle contraction). We used three different imaging modalities (ultrasound of the popliteal artery, BOLD MRI, and near-infrared spectroscopy [NIRS]) and two different exercise intensities (2 and 6 kg). Six healthy men underwent three separate protocols of dynamic plantar flexion exercise on separate days and acute physiological responses were measured. Ultrasound studies showed the percent change in popliteal velocity from baseline to the end of exercise was 151 ± 24% during 2 kg and 589 ± 145% during 6 kg. MRI studies showed an abrupt decrease in BOLD signal intensity at the onset of 2 kg exercise, indicating deoxygenation. The BOLD signal was further reduced during 6 kg exercise (compared to 2 kg) at 1 min (−4.3 ± 0.7 vs. −1.2 ± 0.4%, P < 0.001). Similarly, the change in the NIRS muscle oxygen saturation in the medial gastrocnemius was −11 ± 4% at 2 kg and −38 ± 11% with 6 kg (P = 0.041). In conclusion, we demonstrate that BOLD signal intensity decreases during plantar flexion and this effect is augmented at higher exercise workloads.

Original languageEnglish (US)
Article numbere13004
JournalPhysiological reports
Volume4
Issue number20
DOIs
StatePublished - Oct 1 2016

Fingerprint

Magnetic Resonance Imaging
Exercise
Oxygen
Muscles
Near-Infrared Spectroscopy
Skeletal Muscle
Popliteal Artery
Muscle Contraction
Workload
Ultrasonography

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Muller, Matthew D. ; Li, Zhijun ; Sica, Christopher T. ; Luck, J. Carter ; Gao, Zhaohui ; Blaha, Cheryl A. ; Cauffman, Aimee E. ; Ross, Amanda J. ; Winkler, Nathan J.R. ; Herr, Michael D. ; Brandt, Kristen ; Wang, Jianli ; Gallagher, David C. ; Karunanayaka, Prasanna ; Vesek, Jeffrey ; Leuenberger, Urs A. ; Yang, Qing X. ; Sinoway, Lawrence I. / Muscle oxygenation during dynamic plantar flexion exercise : combining BOLD MRI with traditional physiological measurements. In: Physiological reports. 2016 ; Vol. 4, No. 20.
@article{75fb8016042c424997ec04e6422e3441,
title = "Muscle oxygenation during dynamic plantar flexion exercise: combining BOLD MRI with traditional physiological measurements",
abstract = "Blood-oxygen-level-dependent magnetic resonance imaging (BOLD MRI) has the potential to quantify skeletal muscle oxygenation with high temporal and high spatial resolution. The purpose of this study was to characterize skeletal muscle BOLD responses during steady-state plantar flexion exercise (i.e., during the brief rest periods between muscle contraction). We used three different imaging modalities (ultrasound of the popliteal artery, BOLD MRI, and near-infrared spectroscopy [NIRS]) and two different exercise intensities (2 and 6 kg). Six healthy men underwent three separate protocols of dynamic plantar flexion exercise on separate days and acute physiological responses were measured. Ultrasound studies showed the percent change in popliteal velocity from baseline to the end of exercise was 151 ± 24{\%} during 2 kg and 589 ± 145{\%} during 6 kg. MRI studies showed an abrupt decrease in BOLD signal intensity at the onset of 2 kg exercise, indicating deoxygenation. The BOLD signal was further reduced during 6 kg exercise (compared to 2 kg) at 1 min (−4.3 ± 0.7 vs. −1.2 ± 0.4{\%}, P < 0.001). Similarly, the change in the NIRS muscle oxygen saturation in the medial gastrocnemius was −11 ± 4{\%} at 2 kg and −38 ± 11{\%} with 6 kg (P = 0.041). In conclusion, we demonstrate that BOLD signal intensity decreases during plantar flexion and this effect is augmented at higher exercise workloads.",
author = "Muller, {Matthew D.} and Zhijun Li and Sica, {Christopher T.} and Luck, {J. Carter} and Zhaohui Gao and Blaha, {Cheryl A.} and Cauffman, {Aimee E.} and Ross, {Amanda J.} and Winkler, {Nathan J.R.} and Herr, {Michael D.} and Kristen Brandt and Jianli Wang and Gallagher, {David C.} and Prasanna Karunanayaka and Jeffrey Vesek and Leuenberger, {Urs A.} and Yang, {Qing X.} and Sinoway, {Lawrence I.}",
year = "2016",
month = "10",
day = "1",
doi = "10.14814/phy2.13004",
language = "English (US)",
volume = "4",
journal = "Physiological Reports",
issn = "2051-817X",
publisher = "John Wiley and Sons Inc.",
number = "20",

}

Muller, MD, Li, Z, Sica, CT, Luck, JC, Gao, Z, Blaha, CA, Cauffman, AE, Ross, AJ, Winkler, NJR, Herr, MD, Brandt, K, Wang, J, Gallagher, DC, Karunanayaka, P, Vesek, J, Leuenberger, UA, Yang, QX & Sinoway, LI 2016, 'Muscle oxygenation during dynamic plantar flexion exercise: combining BOLD MRI with traditional physiological measurements', Physiological reports, vol. 4, no. 20, e13004. https://doi.org/10.14814/phy2.13004

Muscle oxygenation during dynamic plantar flexion exercise : combining BOLD MRI with traditional physiological measurements. / Muller, Matthew D.; Li, Zhijun; Sica, Christopher T.; Luck, J. Carter; Gao, Zhaohui; Blaha, Cheryl A.; Cauffman, Aimee E.; Ross, Amanda J.; Winkler, Nathan J.R.; Herr, Michael D.; Brandt, Kristen; Wang, Jianli; Gallagher, David C.; Karunanayaka, Prasanna; Vesek, Jeffrey; Leuenberger, Urs A.; Yang, Qing X.; Sinoway, Lawrence I.

In: Physiological reports, Vol. 4, No. 20, e13004, 01.10.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Muscle oxygenation during dynamic plantar flexion exercise

T2 - combining BOLD MRI with traditional physiological measurements

AU - Muller, Matthew D.

AU - Li, Zhijun

AU - Sica, Christopher T.

AU - Luck, J. Carter

AU - Gao, Zhaohui

AU - Blaha, Cheryl A.

AU - Cauffman, Aimee E.

AU - Ross, Amanda J.

AU - Winkler, Nathan J.R.

AU - Herr, Michael D.

AU - Brandt, Kristen

AU - Wang, Jianli

AU - Gallagher, David C.

AU - Karunanayaka, Prasanna

AU - Vesek, Jeffrey

AU - Leuenberger, Urs A.

AU - Yang, Qing X.

AU - Sinoway, Lawrence I.

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Blood-oxygen-level-dependent magnetic resonance imaging (BOLD MRI) has the potential to quantify skeletal muscle oxygenation with high temporal and high spatial resolution. The purpose of this study was to characterize skeletal muscle BOLD responses during steady-state plantar flexion exercise (i.e., during the brief rest periods between muscle contraction). We used three different imaging modalities (ultrasound of the popliteal artery, BOLD MRI, and near-infrared spectroscopy [NIRS]) and two different exercise intensities (2 and 6 kg). Six healthy men underwent three separate protocols of dynamic plantar flexion exercise on separate days and acute physiological responses were measured. Ultrasound studies showed the percent change in popliteal velocity from baseline to the end of exercise was 151 ± 24% during 2 kg and 589 ± 145% during 6 kg. MRI studies showed an abrupt decrease in BOLD signal intensity at the onset of 2 kg exercise, indicating deoxygenation. The BOLD signal was further reduced during 6 kg exercise (compared to 2 kg) at 1 min (−4.3 ± 0.7 vs. −1.2 ± 0.4%, P < 0.001). Similarly, the change in the NIRS muscle oxygen saturation in the medial gastrocnemius was −11 ± 4% at 2 kg and −38 ± 11% with 6 kg (P = 0.041). In conclusion, we demonstrate that BOLD signal intensity decreases during plantar flexion and this effect is augmented at higher exercise workloads.

AB - Blood-oxygen-level-dependent magnetic resonance imaging (BOLD MRI) has the potential to quantify skeletal muscle oxygenation with high temporal and high spatial resolution. The purpose of this study was to characterize skeletal muscle BOLD responses during steady-state plantar flexion exercise (i.e., during the brief rest periods between muscle contraction). We used three different imaging modalities (ultrasound of the popliteal artery, BOLD MRI, and near-infrared spectroscopy [NIRS]) and two different exercise intensities (2 and 6 kg). Six healthy men underwent three separate protocols of dynamic plantar flexion exercise on separate days and acute physiological responses were measured. Ultrasound studies showed the percent change in popliteal velocity from baseline to the end of exercise was 151 ± 24% during 2 kg and 589 ± 145% during 6 kg. MRI studies showed an abrupt decrease in BOLD signal intensity at the onset of 2 kg exercise, indicating deoxygenation. The BOLD signal was further reduced during 6 kg exercise (compared to 2 kg) at 1 min (−4.3 ± 0.7 vs. −1.2 ± 0.4%, P < 0.001). Similarly, the change in the NIRS muscle oxygen saturation in the medial gastrocnemius was −11 ± 4% at 2 kg and −38 ± 11% with 6 kg (P = 0.041). In conclusion, we demonstrate that BOLD signal intensity decreases during plantar flexion and this effect is augmented at higher exercise workloads.

UR - http://www.scopus.com/inward/record.url?scp=84994246041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994246041&partnerID=8YFLogxK

U2 - 10.14814/phy2.13004

DO - 10.14814/phy2.13004

M3 - Article

C2 - 27798357

AN - SCOPUS:84994246041

VL - 4

JO - Physiological Reports

JF - Physiological Reports

SN - 2051-817X

IS - 20

M1 - e13004

ER -