Musculoskeletal deformities secondary to neurotomy of the superior trunk of the brachial plexus in neonatal mice

H. M. Kim, Leesa M. Galatz, Rosalina Das, Nikunj Patel, Stavros Thomopoulos

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The developmental course of musculoskeletal deformities in neonatal brachial plexus palsy (NBPP) has not been studied extensively. The goals of this study were to: (1) evaluate a new animal model of NBPP, (2) characterize the development of musculoskeletal abnormalities in paralyzed shoulders, and (3) investigate the expression of myogenic and adipogenic genes in paralyzed rotator cuff muscles. Neonatal mice were divided into neurotomy and sham groups. The neurotomy group underwent surgical transection of the superior trunk of the brachial plexus within 24 h of birth. The sham group underwent the same surgical exposure, but the brachial plexus was left intact. Musculoskeletal deformities were evaluated with radiological and histological assays at 2, 4, 8, 12, and 30 weeks after birth. The supraspinatus muscles of a separate group of mice were used to examine expression of myogenic and adipogenic genes at 8 weeks. The neurotomized forelimbs developed deformities similar to those seen in human NBPP. The deformities progressed with age. The denervated supraspinatus muscles showed intramuscular fat accumulation and upregulation of both myogenic and adipogenic genes compared to the normal. The current study presents a useful animal model for future research examining musculoskeletal changes secondary to neonatal nerve injury.

Original languageEnglish (US)
Pages (from-to)1391-1398
Number of pages8
JournalJournal of Orthopaedic Research
Volume28
Issue number10
DOIs
StatePublished - Oct 2010

Fingerprint

Brachial Plexus
Rotator Cuff
Paralysis
Muscles
Musculoskeletal Abnormalities
Animal Models
Parturition
Genes
Forelimb
Up-Regulation
Fats
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine

Cite this

Kim, H. M. ; Galatz, Leesa M. ; Das, Rosalina ; Patel, Nikunj ; Thomopoulos, Stavros. / Musculoskeletal deformities secondary to neurotomy of the superior trunk of the brachial plexus in neonatal mice. In: Journal of Orthopaedic Research. 2010 ; Vol. 28, No. 10. pp. 1391-1398.
@article{aff48899f29440e6824f164d7c4170ba,
title = "Musculoskeletal deformities secondary to neurotomy of the superior trunk of the brachial plexus in neonatal mice",
abstract = "The developmental course of musculoskeletal deformities in neonatal brachial plexus palsy (NBPP) has not been studied extensively. The goals of this study were to: (1) evaluate a new animal model of NBPP, (2) characterize the development of musculoskeletal abnormalities in paralyzed shoulders, and (3) investigate the expression of myogenic and adipogenic genes in paralyzed rotator cuff muscles. Neonatal mice were divided into neurotomy and sham groups. The neurotomy group underwent surgical transection of the superior trunk of the brachial plexus within 24 h of birth. The sham group underwent the same surgical exposure, but the brachial plexus was left intact. Musculoskeletal deformities were evaluated with radiological and histological assays at 2, 4, 8, 12, and 30 weeks after birth. The supraspinatus muscles of a separate group of mice were used to examine expression of myogenic and adipogenic genes at 8 weeks. The neurotomized forelimbs developed deformities similar to those seen in human NBPP. The deformities progressed with age. The denervated supraspinatus muscles showed intramuscular fat accumulation and upregulation of both myogenic and adipogenic genes compared to the normal. The current study presents a useful animal model for future research examining musculoskeletal changes secondary to neonatal nerve injury.",
author = "Kim, {H. M.} and Galatz, {Leesa M.} and Rosalina Das and Nikunj Patel and Stavros Thomopoulos",
year = "2010",
month = "10",
doi = "10.1002/jor.21128",
language = "English (US)",
volume = "28",
pages = "1391--1398",
journal = "Journal of Orthopaedic Research",
issn = "0736-0266",
publisher = "John Wiley and Sons Inc.",
number = "10",

}

Musculoskeletal deformities secondary to neurotomy of the superior trunk of the brachial plexus in neonatal mice. / Kim, H. M.; Galatz, Leesa M.; Das, Rosalina; Patel, Nikunj; Thomopoulos, Stavros.

In: Journal of Orthopaedic Research, Vol. 28, No. 10, 10.2010, p. 1391-1398.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Musculoskeletal deformities secondary to neurotomy of the superior trunk of the brachial plexus in neonatal mice

AU - Kim, H. M.

AU - Galatz, Leesa M.

AU - Das, Rosalina

AU - Patel, Nikunj

AU - Thomopoulos, Stavros

PY - 2010/10

Y1 - 2010/10

N2 - The developmental course of musculoskeletal deformities in neonatal brachial plexus palsy (NBPP) has not been studied extensively. The goals of this study were to: (1) evaluate a new animal model of NBPP, (2) characterize the development of musculoskeletal abnormalities in paralyzed shoulders, and (3) investigate the expression of myogenic and adipogenic genes in paralyzed rotator cuff muscles. Neonatal mice were divided into neurotomy and sham groups. The neurotomy group underwent surgical transection of the superior trunk of the brachial plexus within 24 h of birth. The sham group underwent the same surgical exposure, but the brachial plexus was left intact. Musculoskeletal deformities were evaluated with radiological and histological assays at 2, 4, 8, 12, and 30 weeks after birth. The supraspinatus muscles of a separate group of mice were used to examine expression of myogenic and adipogenic genes at 8 weeks. The neurotomized forelimbs developed deformities similar to those seen in human NBPP. The deformities progressed with age. The denervated supraspinatus muscles showed intramuscular fat accumulation and upregulation of both myogenic and adipogenic genes compared to the normal. The current study presents a useful animal model for future research examining musculoskeletal changes secondary to neonatal nerve injury.

AB - The developmental course of musculoskeletal deformities in neonatal brachial plexus palsy (NBPP) has not been studied extensively. The goals of this study were to: (1) evaluate a new animal model of NBPP, (2) characterize the development of musculoskeletal abnormalities in paralyzed shoulders, and (3) investigate the expression of myogenic and adipogenic genes in paralyzed rotator cuff muscles. Neonatal mice were divided into neurotomy and sham groups. The neurotomy group underwent surgical transection of the superior trunk of the brachial plexus within 24 h of birth. The sham group underwent the same surgical exposure, but the brachial plexus was left intact. Musculoskeletal deformities were evaluated with radiological and histological assays at 2, 4, 8, 12, and 30 weeks after birth. The supraspinatus muscles of a separate group of mice were used to examine expression of myogenic and adipogenic genes at 8 weeks. The neurotomized forelimbs developed deformities similar to those seen in human NBPP. The deformities progressed with age. The denervated supraspinatus muscles showed intramuscular fat accumulation and upregulation of both myogenic and adipogenic genes compared to the normal. The current study presents a useful animal model for future research examining musculoskeletal changes secondary to neonatal nerve injury.

UR - http://www.scopus.com/inward/record.url?scp=77957200454&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957200454&partnerID=8YFLogxK

U2 - 10.1002/jor.21128

DO - 10.1002/jor.21128

M3 - Article

C2 - 20225283

AN - SCOPUS:77957200454

VL - 28

SP - 1391

EP - 1398

JO - Journal of Orthopaedic Research

JF - Journal of Orthopaedic Research

SN - 0736-0266

IS - 10

ER -