Mushroom sciarid fly, Lycoriella ingenua (Diptera

Sciaridae) adults and larvae vector Mushroom Green Mold (Trichoderma aggressivum ft. aggressivum) spores

Maria Mazin, Rob Harvey, Stefanos Andreadis, John Andrew Pecchia, Kevin Cloonan, Edwin George Rajotte

Research output: Contribution to journalArticle

Abstract

Gravid female mushroom sciarid flies, Lycoriella ingenua (Dufour:1839) (Diptera:Sciaridae), were confirmed in laboratory experiments as vectors for the fungal pathogen Trichoderma aggressivum f. aggressivum (Samuels and Gams), causal agent of mushroom green mold disease in mushroom farming. The gravid females acquired the fungal spores when exposed to T. aggressivum cultures, which was confirmed by scanning electron microscopy, the flies vectored T. aggressivum onto clean agar petri dishes, confirmed by new fungal colonies growing on the media and molecular analysis (PCR). Significantly, more T. aggressivum colonies formed on the dishes when flies were left to vector the pathogen for 30 h, as opposed to 18 h. One gravid female fly was able to initiate up to 32 T. aggressivum colonies in a 0.03 m3 area. Frass deposits of mushroom sciarid fly larva reared on T. aggressivum contained viable spores, detected through fungal subcultures and molecular analysis (PCR), confirming that larvae can also vector the fungus. This study supports the heretofore anecdotal evidence that mushroom sciarid flies are part of green mold disease epidemiology on mushroom farms.

Original languageEnglish (US)
JournalApplied Entomology and Zoology
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

Trichoderma aggressivum
Lycoriella
Sciaridae
mushrooms
molds (fungi)
spores
larvae
gravid females
mushroom growing
frass
fungal spores
pathogens
epidemiology
insect larvae
scanning electron microscopy
agar
farms
fungi

All Science Journal Classification (ASJC) codes

  • Insect Science

Cite this

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title = "Mushroom sciarid fly, Lycoriella ingenua (Diptera: Sciaridae) adults and larvae vector Mushroom Green Mold (Trichoderma aggressivum ft. aggressivum) spores",
abstract = "Gravid female mushroom sciarid flies, Lycoriella ingenua (Dufour:1839) (Diptera:Sciaridae), were confirmed in laboratory experiments as vectors for the fungal pathogen Trichoderma aggressivum f. aggressivum (Samuels and Gams), causal agent of mushroom green mold disease in mushroom farming. The gravid females acquired the fungal spores when exposed to T. aggressivum cultures, which was confirmed by scanning electron microscopy, the flies vectored T. aggressivum onto clean agar petri dishes, confirmed by new fungal colonies growing on the media and molecular analysis (PCR). Significantly, more T. aggressivum colonies formed on the dishes when flies were left to vector the pathogen for 30 h, as opposed to 18 h. One gravid female fly was able to initiate up to 32 T. aggressivum colonies in a 0.03 m3 area. Frass deposits of mushroom sciarid fly larva reared on T. aggressivum contained viable spores, detected through fungal subcultures and molecular analysis (PCR), confirming that larvae can also vector the fungus. This study supports the heretofore anecdotal evidence that mushroom sciarid flies are part of green mold disease epidemiology on mushroom farms.",
author = "Maria Mazin and Rob Harvey and Stefanos Andreadis and Pecchia, {John Andrew} and Kevin Cloonan and Rajotte, {Edwin George}",
year = "2019",
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doi = "10.1007/s13355-019-00632-2",
language = "English (US)",
journal = "Applied Entomology and Zoology",
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T2 - Sciaridae) adults and larvae vector Mushroom Green Mold (Trichoderma aggressivum ft. aggressivum) spores

AU - Mazin, Maria

AU - Harvey, Rob

AU - Andreadis, Stefanos

AU - Pecchia, John Andrew

AU - Cloonan, Kevin

AU - Rajotte, Edwin George

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N2 - Gravid female mushroom sciarid flies, Lycoriella ingenua (Dufour:1839) (Diptera:Sciaridae), were confirmed in laboratory experiments as vectors for the fungal pathogen Trichoderma aggressivum f. aggressivum (Samuels and Gams), causal agent of mushroom green mold disease in mushroom farming. The gravid females acquired the fungal spores when exposed to T. aggressivum cultures, which was confirmed by scanning electron microscopy, the flies vectored T. aggressivum onto clean agar petri dishes, confirmed by new fungal colonies growing on the media and molecular analysis (PCR). Significantly, more T. aggressivum colonies formed on the dishes when flies were left to vector the pathogen for 30 h, as opposed to 18 h. One gravid female fly was able to initiate up to 32 T. aggressivum colonies in a 0.03 m3 area. Frass deposits of mushroom sciarid fly larva reared on T. aggressivum contained viable spores, detected through fungal subcultures and molecular analysis (PCR), confirming that larvae can also vector the fungus. This study supports the heretofore anecdotal evidence that mushroom sciarid flies are part of green mold disease epidemiology on mushroom farms.

AB - Gravid female mushroom sciarid flies, Lycoriella ingenua (Dufour:1839) (Diptera:Sciaridae), were confirmed in laboratory experiments as vectors for the fungal pathogen Trichoderma aggressivum f. aggressivum (Samuels and Gams), causal agent of mushroom green mold disease in mushroom farming. The gravid females acquired the fungal spores when exposed to T. aggressivum cultures, which was confirmed by scanning electron microscopy, the flies vectored T. aggressivum onto clean agar petri dishes, confirmed by new fungal colonies growing on the media and molecular analysis (PCR). Significantly, more T. aggressivum colonies formed on the dishes when flies were left to vector the pathogen for 30 h, as opposed to 18 h. One gravid female fly was able to initiate up to 32 T. aggressivum colonies in a 0.03 m3 area. Frass deposits of mushroom sciarid fly larva reared on T. aggressivum contained viable spores, detected through fungal subcultures and molecular analysis (PCR), confirming that larvae can also vector the fungus. This study supports the heretofore anecdotal evidence that mushroom sciarid flies are part of green mold disease epidemiology on mushroom farms.

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