Mutant B-Raf(V600E) promotes melanoma paracellular transmigration by inducing thrombin-mediated endothelial junction breakdown

Pu Zhang, Shan Feng, Gentao Liu, Heyong Wang, Huifeng Zhu, Qiao Ren, Huiyuan Bai, Changliang Fu, Cheng Dong

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: A prothrombotic state is one of the hallmarks of advanced cancer. Results: B-Raf(V600E)-dependent thrombin release from metastatic melanoma signals focal adherens junction disassembly by triggering VE-cadherin phosphorylation and ubiquitination. Conclusion: Thrombin-mediated junction breakdown allows melanoma extravasation via a paracellular route. Significance: Targeting B-Raf(V600E)-mediated thrombin generation and endothelial barrier dysfunction may represent a novel therapeutic strategy to limit tumor metastasis.

Original languageEnglish (US)
Pages (from-to)2087-2106
Number of pages20
JournalJournal of Biological Chemistry
Volume291
Issue number5
DOIs
StatePublished - Jan 29 2016

Fingerprint

Thrombin
Melanoma
Adherens Junctions
Phosphorylation
Ubiquitination
Tumors
Neoplasms
Neoplasm Metastasis
Therapeutics
cadherin 5

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Zhang, Pu ; Feng, Shan ; Liu, Gentao ; Wang, Heyong ; Zhu, Huifeng ; Ren, Qiao ; Bai, Huiyuan ; Fu, Changliang ; Dong, Cheng. / Mutant B-Raf(V600E) promotes melanoma paracellular transmigration by inducing thrombin-mediated endothelial junction breakdown. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 5. pp. 2087-2106.
@article{bb88ee1a40a84200a3f50868dacb22da,
title = "Mutant B-Raf(V600E) promotes melanoma paracellular transmigration by inducing thrombin-mediated endothelial junction breakdown",
abstract = "Background: A prothrombotic state is one of the hallmarks of advanced cancer. Results: B-Raf(V600E)-dependent thrombin release from metastatic melanoma signals focal adherens junction disassembly by triggering VE-cadherin phosphorylation and ubiquitination. Conclusion: Thrombin-mediated junction breakdown allows melanoma extravasation via a paracellular route. Significance: Targeting B-Raf(V600E)-mediated thrombin generation and endothelial barrier dysfunction may represent a novel therapeutic strategy to limit tumor metastasis.",
author = "Pu Zhang and Shan Feng and Gentao Liu and Heyong Wang and Huifeng Zhu and Qiao Ren and Huiyuan Bai and Changliang Fu and Cheng Dong",
year = "2016",
month = "1",
day = "29",
doi = "10.1074/jbc.M115.696419",
language = "English (US)",
volume = "291",
pages = "2087--2106",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "5",

}

Mutant B-Raf(V600E) promotes melanoma paracellular transmigration by inducing thrombin-mediated endothelial junction breakdown. / Zhang, Pu; Feng, Shan; Liu, Gentao; Wang, Heyong; Zhu, Huifeng; Ren, Qiao; Bai, Huiyuan; Fu, Changliang; Dong, Cheng.

In: Journal of Biological Chemistry, Vol. 291, No. 5, 29.01.2016, p. 2087-2106.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mutant B-Raf(V600E) promotes melanoma paracellular transmigration by inducing thrombin-mediated endothelial junction breakdown

AU - Zhang, Pu

AU - Feng, Shan

AU - Liu, Gentao

AU - Wang, Heyong

AU - Zhu, Huifeng

AU - Ren, Qiao

AU - Bai, Huiyuan

AU - Fu, Changliang

AU - Dong, Cheng

PY - 2016/1/29

Y1 - 2016/1/29

N2 - Background: A prothrombotic state is one of the hallmarks of advanced cancer. Results: B-Raf(V600E)-dependent thrombin release from metastatic melanoma signals focal adherens junction disassembly by triggering VE-cadherin phosphorylation and ubiquitination. Conclusion: Thrombin-mediated junction breakdown allows melanoma extravasation via a paracellular route. Significance: Targeting B-Raf(V600E)-mediated thrombin generation and endothelial barrier dysfunction may represent a novel therapeutic strategy to limit tumor metastasis.

AB - Background: A prothrombotic state is one of the hallmarks of advanced cancer. Results: B-Raf(V600E)-dependent thrombin release from metastatic melanoma signals focal adherens junction disassembly by triggering VE-cadherin phosphorylation and ubiquitination. Conclusion: Thrombin-mediated junction breakdown allows melanoma extravasation via a paracellular route. Significance: Targeting B-Raf(V600E)-mediated thrombin generation and endothelial barrier dysfunction may represent a novel therapeutic strategy to limit tumor metastasis.

UR - http://www.scopus.com/inward/record.url?scp=84957012039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957012039&partnerID=8YFLogxK

U2 - 10.1074/jbc.M115.696419

DO - 10.1074/jbc.M115.696419

M3 - Article

C2 - 26504080

AN - SCOPUS:84957012039

VL - 291

SP - 2087

EP - 2106

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 5

ER -