Mutation and recombination frequencies reveal a biological contrast within strains of Cucumber Mosaic Virus

Justin S. Pita, Viktoriya Morris, Marilyn J. Roossinck

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Recent in planta studies have shown that strains Fny and LS of Cucumber mosaic virus (CMV) display differential genetic diversities, Fny and LS having higher and lower mutation frequencies, respectively (J. S. Pita and M. J. Roossinck, J Virol 87:790-797, 2012 http://dx.doi.org/10.1128/JVI.01891-12). In this article, we show that these virus strains have differential recombination frequencies as well. However, the high-diversity Fny strain is a low-recombination virus, whereas the very-low-diversity LS strain is instead a high-recombination virus. Unlike the mutation frequency that was determined by both RNAs 1 and 2, the control elements of recombination frequency reside predominantly within RNA 2, specifically within the 2a gene. IMPORTANCE: Recombination is an important mechanism in virus evolution that can lead to increased or decreased variation and is a major player in virus speciation events that can lead to emerging viruses. Although viral genomes show very frequent evidence of recombination, details of the mechanism involved in these events are still poorly understood. We show here that the reciprocal effects of high mutation frequency and low recombination frequency (and vice versa) involve the RNA-dependent RNA polymerase of the virus, and we speculate that these evolutionary events are related to differences in processivity for two strains of the same virus.

Original languageEnglish (US)
Pages (from-to)6817-6823
Number of pages7
JournalJournal of Virology
Volume89
Issue number13
DOIs
StatePublished - Jan 1 2015

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Cucumovirus
Cucumber mosaic virus
Mutation Rate
Genetic Recombination
Viruses
mutation
viruses
strain differences
RNA-directed RNA polymerase
RNA
RNA Replicase
Viral Genome
RNA Viruses
Plantae
genetic variation
genome

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

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abstract = "Recent in planta studies have shown that strains Fny and LS of Cucumber mosaic virus (CMV) display differential genetic diversities, Fny and LS having higher and lower mutation frequencies, respectively (J. S. Pita and M. J. Roossinck, J Virol 87:790-797, 2012 http://dx.doi.org/10.1128/JVI.01891-12). In this article, we show that these virus strains have differential recombination frequencies as well. However, the high-diversity Fny strain is a low-recombination virus, whereas the very-low-diversity LS strain is instead a high-recombination virus. Unlike the mutation frequency that was determined by both RNAs 1 and 2, the control elements of recombination frequency reside predominantly within RNA 2, specifically within the 2a gene. IMPORTANCE: Recombination is an important mechanism in virus evolution that can lead to increased or decreased variation and is a major player in virus speciation events that can lead to emerging viruses. Although viral genomes show very frequent evidence of recombination, details of the mechanism involved in these events are still poorly understood. We show here that the reciprocal effects of high mutation frequency and low recombination frequency (and vice versa) involve the RNA-dependent RNA polymerase of the virus, and we speculate that these evolutionary events are related to differences in processivity for two strains of the same virus.",
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Mutation and recombination frequencies reveal a biological contrast within strains of Cucumber Mosaic Virus. / Pita, Justin S.; Morris, Viktoriya; Roossinck, Marilyn J.

In: Journal of Virology, Vol. 89, No. 13, 01.01.2015, p. 6817-6823.

Research output: Contribution to journalArticle

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