Mutational analysis of the Arabidopsis RPS2 disease resistance gene and the corresponding Pseudomonas syringae avrRpt2 avirulence gene

Michael Axtell, Timothy W. McNellis, M. B. Mudgett, C. S. Hsu, B. J. Staskawicz

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Plants have evolved a large number of disease resistance genes that encode proteins containing conserved structural motifs that function to recognize pathogen signals and to initiate defense responses. The Arabidopsis RPS2 gene encodes a protein representative of the nucleotide-binding site-leucine-rich repeat (NBS-LRR) class of plant resistance proteins. RPS2 specifically recognizes Pseudomonas syringae pv. tomato strains expressing the avrRpt2 gene and initiates defense responses to bacteria carrying avrRpt2, including a hypersensitive cell death response (HR). We present an in planta mutagenesis experiment that resulted in the isolation of a series of rps2 and avrRpt2 alleles that disrupt the RPS2-avrRpt2 gene-for-gene interaction. Seven novel avrRpt2 alleles incapable of eliciting an RPS2-dependent HR all encode proteins with lesions in the C-terminal portion of AvrRpt2 previously shown to be sufficient for RPS2 recognition. Ten novel rps2 alleles were characterized with mutations in the NBS and the LRR. Several of these alleles code for point mutations in motifs that are conserved among NBS-LRR resistance genes, including the third LRR, which suggests the importance of these motifs for resistance gene function.

Original languageEnglish (US)
Pages (from-to)181-188
Number of pages8
JournalMolecular Plant-Microbe Interactions
Volume14
Issue number2
DOIs
StatePublished - Jan 1 2001

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Pseudomonas syringae
Disease Resistance
Arabidopsis
disease resistance
Alleles
Genes
alleles
genes
Leucine
leucine
binding sites
Nucleotides
proteins
nucleotides
Binding Sites
Pseudomonas syringae pv. tomato
Plant Proteins
lesions (plant)
Proteins
gene interaction

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science

Cite this

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abstract = "Plants have evolved a large number of disease resistance genes that encode proteins containing conserved structural motifs that function to recognize pathogen signals and to initiate defense responses. The Arabidopsis RPS2 gene encodes a protein representative of the nucleotide-binding site-leucine-rich repeat (NBS-LRR) class of plant resistance proteins. RPS2 specifically recognizes Pseudomonas syringae pv. tomato strains expressing the avrRpt2 gene and initiates defense responses to bacteria carrying avrRpt2, including a hypersensitive cell death response (HR). We present an in planta mutagenesis experiment that resulted in the isolation of a series of rps2 and avrRpt2 alleles that disrupt the RPS2-avrRpt2 gene-for-gene interaction. Seven novel avrRpt2 alleles incapable of eliciting an RPS2-dependent HR all encode proteins with lesions in the C-terminal portion of AvrRpt2 previously shown to be sufficient for RPS2 recognition. Ten novel rps2 alleles were characterized with mutations in the NBS and the LRR. Several of these alleles code for point mutations in motifs that are conserved among NBS-LRR resistance genes, including the third LRR, which suggests the importance of these motifs for resistance gene function.",
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Mutational analysis of the Arabidopsis RPS2 disease resistance gene and the corresponding Pseudomonas syringae avrRpt2 avirulence gene. / Axtell, Michael; McNellis, Timothy W.; Mudgett, M. B.; Hsu, C. S.; Staskawicz, B. J.

In: Molecular Plant-Microbe Interactions, Vol. 14, No. 2, 01.01.2001, p. 181-188.

Research output: Contribution to journalArticle

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