Mutations in the HML E silencer of Saccharomyces cerevisiae yield metastable inheritance of transcriptional repression

D. J. Mahoney, R. Marquardt, G. J. Shei, A. B. Rose, J. R. Broach

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Mating-type genes resident in the silent cassette HML at the left arm of chromosome III are repressed by the action of four SIR gene products, mediated independently through two cis-acting sites, termed the E and I silencers. We have found that in the absence of the I silencer, deletion of any one of three distinct elements within E yields partial derepression of the mating-type genes resident at HML, whereas deletion of any two yields full derepression. These elements correspond to a binding site for the abundant DNA-binding protein RAP1, an autonomous replicating sequence (ARS), and an as yet undistinguished region. From detailed deletion analysis of the E site we conclude that the ARS element contributes to silencer function in a capacity distinct from its role as an initiator of DNA replication. In addition, we find that strains deleted for any one of these elements comprise two genetically identical but phenotypically distinct types of cells: Those with HML apparently fully derepressed, and those with HML apparently completely repressed. These results reinforce the notion that epigenetic inheritance is an intrinsic characteristic of silencer action.

Original languageEnglish (US)
Pages (from-to)605-615
Number of pages11
JournalGenes and Development
Volume5
Issue number4
DOIs
StatePublished - Jan 1 1991

Fingerprint

Saccharomyces cerevisiae
Mutation
Genes
DNA-Binding Proteins
DNA Replication
Epigenomics
Chromosomes
Binding Sites

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

@article{e504a54645634344a8e33e1b319546e9,
title = "Mutations in the HML E silencer of Saccharomyces cerevisiae yield metastable inheritance of transcriptional repression",
abstract = "Mating-type genes resident in the silent cassette HML at the left arm of chromosome III are repressed by the action of four SIR gene products, mediated independently through two cis-acting sites, termed the E and I silencers. We have found that in the absence of the I silencer, deletion of any one of three distinct elements within E yields partial derepression of the mating-type genes resident at HML, whereas deletion of any two yields full derepression. These elements correspond to a binding site for the abundant DNA-binding protein RAP1, an autonomous replicating sequence (ARS), and an as yet undistinguished region. From detailed deletion analysis of the E site we conclude that the ARS element contributes to silencer function in a capacity distinct from its role as an initiator of DNA replication. In addition, we find that strains deleted for any one of these elements comprise two genetically identical but phenotypically distinct types of cells: Those with HML apparently fully derepressed, and those with HML apparently completely repressed. These results reinforce the notion that epigenetic inheritance is an intrinsic characteristic of silencer action.",
author = "Mahoney, {D. J.} and R. Marquardt and Shei, {G. J.} and Rose, {A. B.} and Broach, {J. R.}",
year = "1991",
month = "1",
day = "1",
doi = "10.1101/gad.5.4.605",
language = "English (US)",
volume = "5",
pages = "605--615",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "4",

}

Mutations in the HML E silencer of Saccharomyces cerevisiae yield metastable inheritance of transcriptional repression. / Mahoney, D. J.; Marquardt, R.; Shei, G. J.; Rose, A. B.; Broach, J. R.

In: Genes and Development, Vol. 5, No. 4, 01.01.1991, p. 605-615.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mutations in the HML E silencer of Saccharomyces cerevisiae yield metastable inheritance of transcriptional repression

AU - Mahoney, D. J.

AU - Marquardt, R.

AU - Shei, G. J.

AU - Rose, A. B.

AU - Broach, J. R.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Mating-type genes resident in the silent cassette HML at the left arm of chromosome III are repressed by the action of four SIR gene products, mediated independently through two cis-acting sites, termed the E and I silencers. We have found that in the absence of the I silencer, deletion of any one of three distinct elements within E yields partial derepression of the mating-type genes resident at HML, whereas deletion of any two yields full derepression. These elements correspond to a binding site for the abundant DNA-binding protein RAP1, an autonomous replicating sequence (ARS), and an as yet undistinguished region. From detailed deletion analysis of the E site we conclude that the ARS element contributes to silencer function in a capacity distinct from its role as an initiator of DNA replication. In addition, we find that strains deleted for any one of these elements comprise two genetically identical but phenotypically distinct types of cells: Those with HML apparently fully derepressed, and those with HML apparently completely repressed. These results reinforce the notion that epigenetic inheritance is an intrinsic characteristic of silencer action.

AB - Mating-type genes resident in the silent cassette HML at the left arm of chromosome III are repressed by the action of four SIR gene products, mediated independently through two cis-acting sites, termed the E and I silencers. We have found that in the absence of the I silencer, deletion of any one of three distinct elements within E yields partial derepression of the mating-type genes resident at HML, whereas deletion of any two yields full derepression. These elements correspond to a binding site for the abundant DNA-binding protein RAP1, an autonomous replicating sequence (ARS), and an as yet undistinguished region. From detailed deletion analysis of the E site we conclude that the ARS element contributes to silencer function in a capacity distinct from its role as an initiator of DNA replication. In addition, we find that strains deleted for any one of these elements comprise two genetically identical but phenotypically distinct types of cells: Those with HML apparently fully derepressed, and those with HML apparently completely repressed. These results reinforce the notion that epigenetic inheritance is an intrinsic characteristic of silencer action.

UR - http://www.scopus.com/inward/record.url?scp=0025817136&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025817136&partnerID=8YFLogxK

U2 - 10.1101/gad.5.4.605

DO - 10.1101/gad.5.4.605

M3 - Article

C2 - 2010086

AN - SCOPUS:0025817136

VL - 5

SP - 605

EP - 615

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 4

ER -