Mutations in the recD gene of Escherichia coli that raise the copy number of certain plasmids.

R. Seelke, B. Kline, R. Aleff, Ronald D. Porter, M. S. Shields

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Chromosomal mutants were isolated in which, for several small plasmids, there was an increased amount of either covalently closed circular plasmid DNA or total plasmid DNA or both. The mutations were mapped to recD, which has been shown to affect exonuclease V activity and a variety of plasmid maintenance and replication functions. Our results suggest that rolling-circle plasmid replication can occur in recD mutants and that site-specific recombination can resolve the resulting linear multimers into covalently closed circular plasmid forms.

Original languageEnglish (US)
Pages (from-to)4841-4844
Number of pages4
JournalJournal of Bacteriology
Volume169
Issue number10
DOIs
StatePublished - Jan 1 1987

Fingerprint

Plasmids
Escherichia coli
Mutation
Genes
Exodeoxyribonuclease V
Circular DNA
Genetic Recombination
Maintenance
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Seelke, R. ; Kline, B. ; Aleff, R. ; Porter, Ronald D. ; Shields, M. S. / Mutations in the recD gene of Escherichia coli that raise the copy number of certain plasmids. In: Journal of Bacteriology. 1987 ; Vol. 169, No. 10. pp. 4841-4844.
@article{ac04f66cb37d4fdba19e96682bb82507,
title = "Mutations in the recD gene of Escherichia coli that raise the copy number of certain plasmids.",
abstract = "Chromosomal mutants were isolated in which, for several small plasmids, there was an increased amount of either covalently closed circular plasmid DNA or total plasmid DNA or both. The mutations were mapped to recD, which has been shown to affect exonuclease V activity and a variety of plasmid maintenance and replication functions. Our results suggest that rolling-circle plasmid replication can occur in recD mutants and that site-specific recombination can resolve the resulting linear multimers into covalently closed circular plasmid forms.",
author = "R. Seelke and B. Kline and R. Aleff and Porter, {Ronald D.} and Shields, {M. S.}",
year = "1987",
month = "1",
day = "1",
doi = "10.1128/jb.169.10.4841-4844.1987",
language = "English (US)",
volume = "169",
pages = "4841--4844",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "10",

}

Mutations in the recD gene of Escherichia coli that raise the copy number of certain plasmids. / Seelke, R.; Kline, B.; Aleff, R.; Porter, Ronald D.; Shields, M. S.

In: Journal of Bacteriology, Vol. 169, No. 10, 01.01.1987, p. 4841-4844.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mutations in the recD gene of Escherichia coli that raise the copy number of certain plasmids.

AU - Seelke, R.

AU - Kline, B.

AU - Aleff, R.

AU - Porter, Ronald D.

AU - Shields, M. S.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - Chromosomal mutants were isolated in which, for several small plasmids, there was an increased amount of either covalently closed circular plasmid DNA or total plasmid DNA or both. The mutations were mapped to recD, which has been shown to affect exonuclease V activity and a variety of plasmid maintenance and replication functions. Our results suggest that rolling-circle plasmid replication can occur in recD mutants and that site-specific recombination can resolve the resulting linear multimers into covalently closed circular plasmid forms.

AB - Chromosomal mutants were isolated in which, for several small plasmids, there was an increased amount of either covalently closed circular plasmid DNA or total plasmid DNA or both. The mutations were mapped to recD, which has been shown to affect exonuclease V activity and a variety of plasmid maintenance and replication functions. Our results suggest that rolling-circle plasmid replication can occur in recD mutants and that site-specific recombination can resolve the resulting linear multimers into covalently closed circular plasmid forms.

UR - http://www.scopus.com/inward/record.url?scp=0023428656&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023428656&partnerID=8YFLogxK

U2 - 10.1128/jb.169.10.4841-4844.1987

DO - 10.1128/jb.169.10.4841-4844.1987

M3 - Article

VL - 169

SP - 4841

EP - 4844

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 10

ER -