Myosin chains of myocardial tissue - I. Purification and immunological properties of myosin heavy chains

Joan Wikman-Coffelt, Robert Zelis, Claudia Fenner, Dean T. Mason

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

An antiserum specific to dog myocardial myosin has been developed against highly purified myosin heavy chains. The antiserum is specific for the heavy chains of myosin, giving a single precipitin line in an immunodiffusion assay for either the heavy chains of myosin or native myosin, and does not react with any other myocardial proteins. In such assays myosin acts as a single, uniform antigen. Using this antiserum, a radioimmunoassay has been developed to quantitate myosin in a homogenate of myocardial tissue containing free myosin dissociated from other cellular components.

Original languageEnglish (US)
Pages (from-to)1097-1104
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume51
Issue number4
DOIs
StatePublished - Apr 16 1973

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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