N(τ)-methylhistidine release

Contributions of rat skeletal muscle, GI tract, and skin

Steven Wassner, J. B. Li

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The relative contributions of skeletal muscle, gastrointestinal tract, and skin to urinary N(τ)-methylhistidine (MH) excretion were estimated during in vitro studies using the rat hemicorpus preparation. After 0.5 h of perfusion, MH release into the perfusate was linear for 3 h and averaged 29.8 nmol.h-1.100 g hemicorpus-1. In vivo, 24-h urinary MH excretion averaged 37.3 nmol.h-1.100 g body wt-1. The ratio of soft tissue to skin weight is equal (3.2:1) in the whole rat and in the hemicorpus. The gastrointestinal tract released 16.0 nmol.h-1.100 g body wt-1 or approximately 41% of the total urinary MH excretion. Preparations perfused with or without skin showed modest differences in the rate of MH release that were not statistically significant. Skeletal muscle contains 89.8% of total body MH content, whereas gastrointestinal tract and skin contain 3.8 and 6.4%, respectively. Gastrointestinal tract actomyosin turns over rapidly with a fractional catabolic rate of 24%/day versus 1.4%/day for skeletal muscle actomyosin.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume6
Issue number4
StatePublished - Jan 1 1982

Fingerprint

Methylhistidines
Gastrointestinal Tract
Skeletal Muscle
Skin
Actomyosin
Perfusion
Weights and Measures

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

@article{c501021d659749178d03dcd10d7e8419,
title = "N(τ)-methylhistidine release: Contributions of rat skeletal muscle, GI tract, and skin",
abstract = "The relative contributions of skeletal muscle, gastrointestinal tract, and skin to urinary N(τ)-methylhistidine (MH) excretion were estimated during in vitro studies using the rat hemicorpus preparation. After 0.5 h of perfusion, MH release into the perfusate was linear for 3 h and averaged 29.8 nmol.h-1.100 g hemicorpus-1. In vivo, 24-h urinary MH excretion averaged 37.3 nmol.h-1.100 g body wt-1. The ratio of soft tissue to skin weight is equal (3.2:1) in the whole rat and in the hemicorpus. The gastrointestinal tract released 16.0 nmol.h-1.100 g body wt-1 or approximately 41{\%} of the total urinary MH excretion. Preparations perfused with or without skin showed modest differences in the rate of MH release that were not statistically significant. Skeletal muscle contains 89.8{\%} of total body MH content, whereas gastrointestinal tract and skin contain 3.8 and 6.4{\%}, respectively. Gastrointestinal tract actomyosin turns over rapidly with a fractional catabolic rate of 24{\%}/day versus 1.4{\%}/day for skeletal muscle actomyosin.",
author = "Steven Wassner and Li, {J. B.}",
year = "1982",
month = "1",
day = "1",
language = "English (US)",
volume = "6",
journal = "American Journal of Physiology",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - N(τ)-methylhistidine release

T2 - Contributions of rat skeletal muscle, GI tract, and skin

AU - Wassner, Steven

AU - Li, J. B.

PY - 1982/1/1

Y1 - 1982/1/1

N2 - The relative contributions of skeletal muscle, gastrointestinal tract, and skin to urinary N(τ)-methylhistidine (MH) excretion were estimated during in vitro studies using the rat hemicorpus preparation. After 0.5 h of perfusion, MH release into the perfusate was linear for 3 h and averaged 29.8 nmol.h-1.100 g hemicorpus-1. In vivo, 24-h urinary MH excretion averaged 37.3 nmol.h-1.100 g body wt-1. The ratio of soft tissue to skin weight is equal (3.2:1) in the whole rat and in the hemicorpus. The gastrointestinal tract released 16.0 nmol.h-1.100 g body wt-1 or approximately 41% of the total urinary MH excretion. Preparations perfused with or without skin showed modest differences in the rate of MH release that were not statistically significant. Skeletal muscle contains 89.8% of total body MH content, whereas gastrointestinal tract and skin contain 3.8 and 6.4%, respectively. Gastrointestinal tract actomyosin turns over rapidly with a fractional catabolic rate of 24%/day versus 1.4%/day for skeletal muscle actomyosin.

AB - The relative contributions of skeletal muscle, gastrointestinal tract, and skin to urinary N(τ)-methylhistidine (MH) excretion were estimated during in vitro studies using the rat hemicorpus preparation. After 0.5 h of perfusion, MH release into the perfusate was linear for 3 h and averaged 29.8 nmol.h-1.100 g hemicorpus-1. In vivo, 24-h urinary MH excretion averaged 37.3 nmol.h-1.100 g body wt-1. The ratio of soft tissue to skin weight is equal (3.2:1) in the whole rat and in the hemicorpus. The gastrointestinal tract released 16.0 nmol.h-1.100 g body wt-1 or approximately 41% of the total urinary MH excretion. Preparations perfused with or without skin showed modest differences in the rate of MH release that were not statistically significant. Skeletal muscle contains 89.8% of total body MH content, whereas gastrointestinal tract and skin contain 3.8 and 6.4%, respectively. Gastrointestinal tract actomyosin turns over rapidly with a fractional catabolic rate of 24%/day versus 1.4%/day for skeletal muscle actomyosin.

UR - http://www.scopus.com/inward/record.url?scp=0020201812&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020201812&partnerID=8YFLogxK

M3 - Article

VL - 6

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0193-1849

IS - 4

ER -