N-3 fatty acids and chylomicron metabolism in the rat

William S. Harris, Bo E. Hustvedt, Egil Hagen, Michael Henry Green, Guoping Lu, Christian A. Drevon

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Dietary n-3 fatty acids (FAs) reduce postprandial triacylglycerol concentrations in humans by unknown mechanisms. Our goals were to reproduce this phenomenon in the rat, and then to determine the mechanism. In an oral fat tolerance study two groups of rats were fed diets containing 2.1% ethyl esters of n-3 FA or olive oil for 2 weeks. After gavaging with emulsified soybean oil, the postprandial chylomicron triacylglycerol levels in the n-3 FA group were reduced by 40% (P < 0.05). The hypothesis that n-3 FA feeding reduced chylomicron production/secretion from the gut was tested by blocking chylomicron removal with Triton WR1339 before gavaging the rats with the fat load. This completely eliminated the hypochylomicronemic effect suggesting that chylomicron input was not inhibited by n-3 FAs. Chylomicron clearance was studied by injecting chylomicrons containing radioactive retinyl esters and triacylglycerol into rats from both groups. Pre-feeding with n-3 FAs accelerated the removal of chylomicron triacylglycerol and retinyl esters from the plasma with significantly lower fractions of dose remaining at 2, 4, and 8 min post-injection for both tracers. This findings suggest that n-3 FAs reduce postprandial chylomicronemia in the rat by accelerating chylomicron lipid clearance.

Original languageEnglish (US)
Pages (from-to)503-515
Number of pages13
JournalJournal of Lipid Research
Volume38
Issue number3
StatePublished - Mar 1 1997

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Chylomicrons
Omega-3 Fatty Acids
Metabolism
Rats
Triglycerides
Esters
Fats
Soybean Oil
Nutrition
Diet
Lipids
Plasmas
Injections

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Harris, W. S., Hustvedt, B. E., Hagen, E., Green, M. H., Lu, G., & Drevon, C. A. (1997). N-3 fatty acids and chylomicron metabolism in the rat. Journal of Lipid Research, 38(3), 503-515.
Harris, William S. ; Hustvedt, Bo E. ; Hagen, Egil ; Green, Michael Henry ; Lu, Guoping ; Drevon, Christian A. / N-3 fatty acids and chylomicron metabolism in the rat. In: Journal of Lipid Research. 1997 ; Vol. 38, No. 3. pp. 503-515.
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Harris, WS, Hustvedt, BE, Hagen, E, Green, MH, Lu, G & Drevon, CA 1997, 'N-3 fatty acids and chylomicron metabolism in the rat', Journal of Lipid Research, vol. 38, no. 3, pp. 503-515.

N-3 fatty acids and chylomicron metabolism in the rat. / Harris, William S.; Hustvedt, Bo E.; Hagen, Egil; Green, Michael Henry; Lu, Guoping; Drevon, Christian A.

In: Journal of Lipid Research, Vol. 38, No. 3, 01.03.1997, p. 503-515.

Research output: Contribution to journalArticle

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Harris WS, Hustvedt BE, Hagen E, Green MH, Lu G, Drevon CA. N-3 fatty acids and chylomicron metabolism in the rat. Journal of Lipid Research. 1997 Mar 1;38(3):503-515.