N-cadherin levels in endothelial cells are regulated by monolayer maturity and p120 availability.

Deana M. Ferreri, Fred L. Minnear, Taofei Yin, Andrew P. Kowalczyk, Peter A. Vincent

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Endothelial cells (ECs) express VE-cadherin and N-cadherin, and recent data suggest that VE-cadherin levels are dependent on N-cadherin expression. While investigating changes in N-cadherin levels during endothelial monolayer maturation, the authors found that VE-cadherin levels are maintained in ECs despite a decrease in N-cadherin, suggesting that VE-cadherin levels may not depend on N-cadherin. Knockdown of N-cadherin did not affect VE-cadherin levels in ECs with low endogenous N-cadherin expression. Surprisingly, however, knockdown of N-cadherin in ECs with high endogenous N-cadherin expression increased VE-cadherin levels, suggesting an inverse relationship between the two. This was further supported by a decrease in VE-cadherin following overexpression of N-cadherin. Experiments in which p120, a catenin that binds N- and VE-cadherin, was knocked down or overexpressed indicate that these two cadherins compete for p120. These data demonstrate that VE-cadherin levels are not directly related to N-cadherin levels but may be inversely related due to competition for p120.

Original languageEnglish (US)
Pages (from-to)333-349
Number of pages17
JournalCell Communication and Adhesion
Volume15
Issue number4
DOIs
StatePublished - Nov 2008

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology

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