N-linked oligosaccharides of cobra venom factor novel α(1-3)galactosylated LeX structures

D. Channe Gowda, John Glushka, Herman Van Halbeek, Rao N. Thotakura, Reinhard Bredehorst, Carl Wilhelm Vogel

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Cobra venom factor (CVF), a nontoxic, complement-activating glycoprotein in cobra venom, is a functional analog of mammalian complement component C3b. The carbohydrate moiety of CVF consists exclusively of N-linked oligosaccharides with terminal α1-3-linked galactosyl residues, which are antigenic in human. CVF has potential for several medical applications, including targeted cell killing and complement depletion. Here, we report a detailed structural analysis of the oligosaccharides of CVF. The structures of the oligosaccharides were determined by lectin affinity chromatography, antibody affinity blotting, compositional and methylation analyses, and high-resolution 1H-NMR spectroscopy. Approximately 80% of the oligosaccharides are diantennary complex-type, Ο12% are tri- and tetra-antennary complex-type, and Ο8% are oligomannose type structures. The majority of the complex-type oligosaccharides terminate in Galα1-3Galβ1-4(Fucα1-3)GlcNAcβ1, a unique carbohydrate structural feature abundantly present in the glycoproteins of cobra venom.

Original languageEnglish (US)
Pages (from-to)195-208
Number of pages14
JournalGlycobiology
Volume11
Issue number3
DOIs
StatePublished - Mar 2001

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Oligosaccharides
Cobra Venoms
Glycoproteins
Carbohydrates
Complement C3b
Affinity chromatography
Methylation
Antibody Affinity
Medical applications
Affinity Chromatography
Lectins
Structural analysis
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Cells
cobra venom factor
Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Gowda, D. C., Glushka, J., Van Halbeek, H., Thotakura, R. N., Bredehorst, R., & Vogel, C. W. (2001). N-linked oligosaccharides of cobra venom factor novel α(1-3)galactosylated LeX structures. Glycobiology, 11(3), 195-208. https://doi.org/10.1093/glycob/11.3.195
Gowda, D. Channe ; Glushka, John ; Van Halbeek, Herman ; Thotakura, Rao N. ; Bredehorst, Reinhard ; Vogel, Carl Wilhelm. / N-linked oligosaccharides of cobra venom factor novel α(1-3)galactosylated LeX structures. In: Glycobiology. 2001 ; Vol. 11, No. 3. pp. 195-208.
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Gowda, DC, Glushka, J, Van Halbeek, H, Thotakura, RN, Bredehorst, R & Vogel, CW 2001, 'N-linked oligosaccharides of cobra venom factor novel α(1-3)galactosylated LeX structures', Glycobiology, vol. 11, no. 3, pp. 195-208. https://doi.org/10.1093/glycob/11.3.195

N-linked oligosaccharides of cobra venom factor novel α(1-3)galactosylated LeX structures. / Gowda, D. Channe; Glushka, John; Van Halbeek, Herman; Thotakura, Rao N.; Bredehorst, Reinhard; Vogel, Carl Wilhelm.

In: Glycobiology, Vol. 11, No. 3, 03.2001, p. 195-208.

Research output: Contribution to journalArticle

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