N-linked oligosaccharides of cobra venom factor novel α(1-3)galactosylated LeX structures

D. Channe Gowda, John Glushka, Herman Van Halbeek, Rao N. Thotakura, Reinhard Bredehorst, Carl Wilhelm Vogel

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Cobra venom factor (CVF), a nontoxic, complement-activating glycoprotein in cobra venom, is a functional analog of mammalian complement component C3b. The carbohydrate moiety of CVF consists exclusively of N-linked oligosaccharides with terminal α1-3-linked galactosyl residues, which are antigenic in human. CVF has potential for several medical applications, including targeted cell killing and complement depletion. Here, we report a detailed structural analysis of the oligosaccharides of CVF. The structures of the oligosaccharides were determined by lectin affinity chromatography, antibody affinity blotting, compositional and methylation analyses, and high-resolution 1H-NMR spectroscopy. Approximately 80% of the oligosaccharides are diantennary complex-type, Ο12% are tri- and tetra-antennary complex-type, and Ο8% are oligomannose type structures. The majority of the complex-type oligosaccharides terminate in Galα1-3Galβ1-4(Fucα1-3)GlcNAcβ1, a unique carbohydrate structural feature abundantly present in the glycoproteins of cobra venom.

Original languageEnglish (US)
Pages (from-to)195-208
Number of pages14
JournalGlycobiology
Volume11
Issue number3
DOIs
StatePublished - Mar 2001

All Science Journal Classification (ASJC) codes

  • Biochemistry

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    Gowda, D. C., Glushka, J., Van Halbeek, H., Thotakura, R. N., Bredehorst, R., & Vogel, C. W. (2001). N-linked oligosaccharides of cobra venom factor novel α(1-3)galactosylated LeX structures. Glycobiology, 11(3), 195-208. https://doi.org/10.1093/glycob/11.3.195