NaCl-induced CsRCI2E and CsRCI2F interact with aquaporin CsPIP2;1 to reduce water transport in Camelina sativa L.

Hyun Sung Kim, Won Park, Hyun Gyu Lim, Sanung Eom, Jun Ho Lee, John Edward Carlson, Sung Ju Ahn

Research output: Contribution to journalArticle

Abstract

Rare cold-inducible 2 (RCI2) proteins are small hydrophobic proteins that are known to be localized in cellular membranes. The function of RCI2 proteins has been reported to be associated with low-temperature, salt, and drought stress tolerances as a membrane potential regulator; however, the specific functions are still unknown. The PIP2 (plasma membrane intrinsic protein 2) aquaporins are proteins that transport water and small solutes into the cell. The expression and activity of PIP2 proteins, like RCI2, are also related to salt- and drought-stress tolerance. In this study, we identified novel protein interactions between RCI2 and PIP2; 1, including protein accumulation changes in the bioenergy crop Camelina sativa L. under various NaCl stress conditions. Accumulation of both CsRCI2E and CsRCI2F proteins increased with NaCl stress; however, to differing levels depending on the NaCl stress intensity. A co-immunoprecipitation test revealed interaction between CsRCI2E-CsPIP2 and CsRCI2F-CsPIP2. Moreover, co-expression of the four CsRCI2 proteins with CsPIP2; 1 in Xenopus laevis oocytes reduced water transport activity. Furthermore, the abundance of CsPIP2; 1 protein was decreased under CsRCI2E and CsRCI2F co-expression. These results suggest that NaCl-induced expression of CsRCI2E and CsRCI2F contributes to the regulation of CsPIP2; 1.

Original languageEnglish (US)
Pages (from-to)213-218
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume513
Issue number1
DOIs
StatePublished - May 21 2019

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Aquaporin 1
Water
Proteins
Blood Proteins
Membrane Proteins
Droughts
Cell Membrane
Cell membranes
Drought
Salts
Aquaporin 2
Xenopus laevis
Protein Transport
Immunoprecipitation
Membrane Potentials
Membranes
Oocytes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kim, Hyun Sung ; Park, Won ; Lim, Hyun Gyu ; Eom, Sanung ; Lee, Jun Ho ; Carlson, John Edward ; Ahn, Sung Ju. / NaCl-induced CsRCI2E and CsRCI2F interact with aquaporin CsPIP2;1 to reduce water transport in Camelina sativa L. In: Biochemical and Biophysical Research Communications. 2019 ; Vol. 513, No. 1. pp. 213-218.
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abstract = "Rare cold-inducible 2 (RCI2) proteins are small hydrophobic proteins that are known to be localized in cellular membranes. The function of RCI2 proteins has been reported to be associated with low-temperature, salt, and drought stress tolerances as a membrane potential regulator; however, the specific functions are still unknown. The PIP2 (plasma membrane intrinsic protein 2) aquaporins are proteins that transport water and small solutes into the cell. The expression and activity of PIP2 proteins, like RCI2, are also related to salt- and drought-stress tolerance. In this study, we identified novel protein interactions between RCI2 and PIP2; 1, including protein accumulation changes in the bioenergy crop Camelina sativa L. under various NaCl stress conditions. Accumulation of both CsRCI2E and CsRCI2F proteins increased with NaCl stress; however, to differing levels depending on the NaCl stress intensity. A co-immunoprecipitation test revealed interaction between CsRCI2E-CsPIP2 and CsRCI2F-CsPIP2. Moreover, co-expression of the four CsRCI2 proteins with CsPIP2; 1 in Xenopus laevis oocytes reduced water transport activity. Furthermore, the abundance of CsPIP2; 1 protein was decreased under CsRCI2E and CsRCI2F co-expression. These results suggest that NaCl-induced expression of CsRCI2E and CsRCI2F contributes to the regulation of CsPIP2; 1.",
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NaCl-induced CsRCI2E and CsRCI2F interact with aquaporin CsPIP2;1 to reduce water transport in Camelina sativa L. / Kim, Hyun Sung; Park, Won; Lim, Hyun Gyu; Eom, Sanung; Lee, Jun Ho; Carlson, John Edward; Ahn, Sung Ju.

In: Biochemical and Biophysical Research Communications, Vol. 513, No. 1, 21.05.2019, p. 213-218.

Research output: Contribution to journalArticle

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T1 - NaCl-induced CsRCI2E and CsRCI2F interact with aquaporin CsPIP2;1 to reduce water transport in Camelina sativa L.

AU - Kim, Hyun Sung

AU - Park, Won

AU - Lim, Hyun Gyu

AU - Eom, Sanung

AU - Lee, Jun Ho

AU - Carlson, John Edward

AU - Ahn, Sung Ju

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