Nanoindentation of Isometric Viruses on Deterministically Corrugated Substrates

M. Hernando-Pérez, C. Zeng, L. Delalande, I. B. Tsvetkova, Arthur Bousquet, M. Tayachi-Pigeonnat, R. Temam, B. Dragnea

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

It has been just over 100 years since inventor Joseph Coyle perfected the egg carton-a package format that has known very little changes since its first appearance (Dhillon, S. B. C. Inventor Created Better Way to Carry Eggs. In The Globe and Mail Vancouver, 2013). In this article, we extend Coyles old idea to the study of mechanical properties of viruses. Virus stiffness, strength, and breaking force obtained by force spectroscopy atomic force microscopy (AFM) provide the knowledge required for designing nanocontainers for applications in biotechnology and medicine, and for understanding the fundamentals of virus-host interaction such as virus translocation from one cellular compartment to another. In previous studies, virus particles adsorbed on flat surfaces from a physiological buffer were subjected to directional deformation by a known force exerted via a microscopic probe. The affinity between the virus shell and surface is required to be strong enough to anchor particles on the substrate while they are indented or imaged, yet sufficiently weak to preserve the native structure and interactions prior deformation. The specific question addressed here is whether an experimental scheme characterized by increased contact area and stable mechanical equilibrium under directional compression would provide a more reliable characterization than the traditional flat substrate approach.

Original languageEnglish (US)
Pages (from-to)340-347
Number of pages8
JournalJournal of Physical Chemistry B
Volume120
Issue number2
DOIs
StatePublished - Jan 21 2016

Fingerprint

viruses
Nanoindentation
nanoindentation
Viruses
Substrates
eggs
biotechnology
globes
compartments
Biotechnology
Anchors
medicine
Medicine
format
affinity
flat surfaces
Atomic force microscopy
stiffness
Buffers
buffers

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Hernando-Pérez, M., Zeng, C., Delalande, L., Tsvetkova, I. B., Bousquet, A., Tayachi-Pigeonnat, M., ... Dragnea, B. (2016). Nanoindentation of Isometric Viruses on Deterministically Corrugated Substrates. Journal of Physical Chemistry B, 120(2), 340-347. https://doi.org/10.1021/acs.jpcb.5b08362
Hernando-Pérez, M. ; Zeng, C. ; Delalande, L. ; Tsvetkova, I. B. ; Bousquet, Arthur ; Tayachi-Pigeonnat, M. ; Temam, R. ; Dragnea, B. / Nanoindentation of Isometric Viruses on Deterministically Corrugated Substrates. In: Journal of Physical Chemistry B. 2016 ; Vol. 120, No. 2. pp. 340-347.
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Hernando-Pérez, M, Zeng, C, Delalande, L, Tsvetkova, IB, Bousquet, A, Tayachi-Pigeonnat, M, Temam, R & Dragnea, B 2016, 'Nanoindentation of Isometric Viruses on Deterministically Corrugated Substrates', Journal of Physical Chemistry B, vol. 120, no. 2, pp. 340-347. https://doi.org/10.1021/acs.jpcb.5b08362

Nanoindentation of Isometric Viruses on Deterministically Corrugated Substrates. / Hernando-Pérez, M.; Zeng, C.; Delalande, L.; Tsvetkova, I. B.; Bousquet, Arthur; Tayachi-Pigeonnat, M.; Temam, R.; Dragnea, B.

In: Journal of Physical Chemistry B, Vol. 120, No. 2, 21.01.2016, p. 340-347.

Research output: Contribution to journalArticle

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AU - Temam, R.

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Hernando-Pérez M, Zeng C, Delalande L, Tsvetkova IB, Bousquet A, Tayachi-Pigeonnat M et al. Nanoindentation of Isometric Viruses on Deterministically Corrugated Substrates. Journal of Physical Chemistry B. 2016 Jan 21;120(2):340-347. https://doi.org/10.1021/acs.jpcb.5b08362