Current methods for the extended culture of human embryonic stem cells (hESCs) are far from optimal. Therefore, we proposed to use nanoporous membrane for the co-culture of feeder cells and hESCs. In this proof-of-concept study, we first studied the stability, biocompatibility and permeability of an alginate-poly-L-lysine-alginate membrane. This membrane was then used to encapsulate feeder cells to examine the cell viability and TGF-ß secretion. Finally, the encapsulated feeder-conditioned media were used to culture hESCs. The data indicate that nanoporous membrane is a potential tool for the co-culture of feeder cells and hESCs in a safe, efficient, and continuous manner.
|Original language||English (US)|
|Number of pages||12|
|Journal||International Journal of Functional Informatics and Personalised Medicine|
|State||Published - Jan 1 2009|
All Science Journal Classification (ASJC) codes
- Clinical Neurology