Nanoporous membrane-encapsulated feeder cells for culture of human embryonic stem cells

Jing Zhou, Ren He Xu, Yong Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Current methods for the extended culture of human embryonic stem cells (hESCs) are far from optimal. Therefore, we proposed to use nanoporous membrane for the co-culture of feeder cells and hESCs. In this proof-of-concept study, we first studied the stability, biocompatibility and permeability of an alginate-poly-L-lysine-alginate membrane. This membrane was then used to encapsulate feeder cells to examine the cell viability and TGF-ß secretion. Finally, the encapsulated feeder-conditioned media were used to culture hESCs. The data indicate that nanoporous membrane is a potential tool for the co-culture of feeder cells and hESCs in a safe, efficient, and continuous manner.

Original languageEnglish (US)
Pages (from-to)77-88
Number of pages12
JournalInternational Journal of Functional Informatics and Personalised Medicine
Volume2
Issue number1
DOIs
StatePublished - 2009

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Feeder Cells
Cell Culture Techniques
Membranes
Coculture Techniques
Conditioned Culture Medium
Permeability
Cell Survival
Human Embryonic Stem Cells
alginate-polylysine-alginate

All Science Journal Classification (ASJC) codes

  • Clinical Neurology

Cite this

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abstract = "Current methods for the extended culture of human embryonic stem cells (hESCs) are far from optimal. Therefore, we proposed to use nanoporous membrane for the co-culture of feeder cells and hESCs. In this proof-of-concept study, we first studied the stability, biocompatibility and permeability of an alginate-poly-L-lysine-alginate membrane. This membrane was then used to encapsulate feeder cells to examine the cell viability and TGF-{\ss} secretion. Finally, the encapsulated feeder-conditioned media were used to culture hESCs. The data indicate that nanoporous membrane is a potential tool for the co-culture of feeder cells and hESCs in a safe, efficient, and continuous manner.",
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