Nanoscale extracellular vesicle-derived DNA is superior to circulating cell-free DNA for mutation detection in early-stage non-small-cell lung cancer

Y. Wan, B. Liu, H. Lei, B. Zhang, Y. Wang, H. Huang, S. Chen, Y. Feng, L. Zhu, Y. Gu, Q. Zhang, H. Ma, S. Y. Zheng

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Abstract

Background: The comparison between relatively intact nanoscale extracellular vesicle-derived DNA (nEV-DNA) and fragmented circulating cell-free DNA (cfDNA) in mutation detection among patients with non-small-cell lung cancer (NSCLC) has not been carried out yet, and thus deserves investigation. Patients and methods: Both nEV-DNA and cfDNA was obtained from 377 NSCLC patients with known EGFR mutation status and 69 controls. The respective EGFR E19del/T790M/L858R mutation status was interrogated with amplification-refractory-mutation-system-based PCR assays (ARMS-PCR). Results: Neither nEV-DNA nor cfDNA levels show a strong correlation with tumor volumes. There is no correlation between cfDNA and nEV-DNA levels either. The detection sensitivity of nEV-DNA and cfDNA using ARMS-PCR in early-stage NSCLC was 25.7% and 14.2%, respectively, with 96.6% and 91.7% specificity, respectively. In late-stage NSCLC, both nEV-DNA and cfDNA show 80% sensitivity and over 95% specificity. Conclusions: nEV-DNA is superior to cfDNA for mutation detection in early-stage NSCLC using ARMS-PCR. However, the advantages vanish in late-stage NSCLC.

Original languageEnglish (US)
Pages (from-to)2379-2383
Number of pages5
JournalAnnals of Oncology
Volume29
Issue number12
DOIs
StatePublished - Jan 1 2018

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All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology

Cite this

Wan, Y., Liu, B., Lei, H., Zhang, B., Wang, Y., Huang, H., Chen, S., Feng, Y., Zhu, L., Gu, Y., Zhang, Q., Ma, H., & Zheng, S. Y. (2018). Nanoscale extracellular vesicle-derived DNA is superior to circulating cell-free DNA for mutation detection in early-stage non-small-cell lung cancer. Annals of Oncology, 29(12), 2379-2383. https://doi.org/10.1093/annonc/mdy458