TY - JOUR
T1 - Narrowly confined and glomerulus-specific onset latencies of odor-evoked calcium transients in the juxtaglomerular cells of the mouse main olfactory bulb
AU - Homma, Ryota
AU - Lv, Xiaohua
AU - Sato, Tokiharu
AU - Imamura, Fumiaki
AU - Zeng, Shaoqun
AU - Nagayama, Shin
N1 - Funding Information:
Received October 2, 2018; accepted February 5, 2019; First published February 11, 2019. The authors declare no competing financial interests. Author contributions: R.H. and S.N. designed research; R.H., X.L., T.S., F.I., S.Z., and S.N. performed research; R.H. analyzed data; R.H. and S.N. wrote the paper. This work was supported by the NIH, National Institute on Deafness and Other Communication Disorders (Grants R01DC013802 and R01DC016307) and National Natural Science Foundation of China (Grants 81327802 and 61721092). We thank Wei R. Chen for his contribution to the setup of the acousto-optic deflector two-photon microscope. *RH and XL contributed equally to this work. Correspondence should be addressed to Shin Nagayama at Shin.Nagayama@ uth.tmc.edu or Ryota Homma at Ryota.Homma@uth.tmc.edu. https://doi.org/10.1523/ENEURO.0387-18.2019 Copyright © 2019 Homma et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Publisher Copyright:
© 2019 Homma et al.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Odor information is transmitted from olfactory sensory neurons to principal neurons at the glomeruli of the olfactory bulb. The intraglomerular neuronal circuit also includes hundreds of interneurons referred to as juxtaglomerular (JG) cells. Stimulus selectivity is well correlated among many JG cells that are associated with the same glomerulus, consistent with their highly homogeneous sensory inputs. However, much less is known about the temporal aspects of their activity, including the temporal coordination of their odor-evoked responses. As many JG cells within a glomerular module respond to the same stimulus, the extent to which their activity is temporally aligned will affect the temporal profile of their population inhibitory inputs. Using random-access high-speed two-photon microscopy, we recorded the odor-evoked calcium transients of mouse JG cells and compared the onset latency and rise time among neurons putatively associated with the same and different glomeruli. Whereas the overall onset latencies of odorevoked transients were distributed across a ~150 ms time window, those from cells putatively associated with the same glomerulus were confined to a much narrower window of several tens of milliseconds. This result suggests that onset latency primarily depends on the associated glomerulus. We also observed glomerular specificity in the rise time. The glomerulus-specific temporal pattern of odor-evoked activity implies that the temporal patterns of inputs from the intraglomerular circuit are unique to individual glomerulusodor pairs, which may contribute to efficient shaping of the temporal pattern of activity in the principal neurons.
AB - Odor information is transmitted from olfactory sensory neurons to principal neurons at the glomeruli of the olfactory bulb. The intraglomerular neuronal circuit also includes hundreds of interneurons referred to as juxtaglomerular (JG) cells. Stimulus selectivity is well correlated among many JG cells that are associated with the same glomerulus, consistent with their highly homogeneous sensory inputs. However, much less is known about the temporal aspects of their activity, including the temporal coordination of their odor-evoked responses. As many JG cells within a glomerular module respond to the same stimulus, the extent to which their activity is temporally aligned will affect the temporal profile of their population inhibitory inputs. Using random-access high-speed two-photon microscopy, we recorded the odor-evoked calcium transients of mouse JG cells and compared the onset latency and rise time among neurons putatively associated with the same and different glomeruli. Whereas the overall onset latencies of odorevoked transients were distributed across a ~150 ms time window, those from cells putatively associated with the same glomerulus were confined to a much narrower window of several tens of milliseconds. This result suggests that onset latency primarily depends on the associated glomerulus. We also observed glomerular specificity in the rise time. The glomerulus-specific temporal pattern of odor-evoked activity implies that the temporal patterns of inputs from the intraglomerular circuit are unique to individual glomerulusodor pairs, which may contribute to efficient shaping of the temporal pattern of activity in the principal neurons.
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U2 - 10.1523/ENEURO.0387-18.2019
DO - 10.1523/ENEURO.0387-18.2019
M3 - Article
C2 - 30834302
AN - SCOPUS:85062421001
VL - 6
JO - eNeuro
JF - eNeuro
SN - 2373-2822
IS - 1
M1 - e0387-18.2019
ER -