Native human papillomavirus production, quantification, and infectivity analysis

Jennifer Biryukov, Linda Cruz, Eric J. Ryndock, Craig Meyers

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

In a natural infection, human papillomavirus (HPV) replicates in a stratified and differentiated epithelium. We have developed an in vitro organotypic raft culture system that allows researchers to study HPV in its natural environment. Not only does this system reproduce the differentiation-dependent replication cycle of HPV, but it also allows for the production of high titers of native HPV virions. Currently, much of the HPV research has been done utilizing synthetic particles produced in transfection systems. However, by production of native virions, this research can now be continued using native particles. This chapter presents methods for producing, titering, and qualitating, via infectivity assay, native virus produced from organotypic raft culture.

Original languageEnglish (US)
Title of host publicationCervical Cancer
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Pages317-331
Number of pages15
ISBN (Electronic)9781493920136
ISBN (Print)9781493920129
DOIs
StatePublished - Oct 27 2014

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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    Biryukov, J., Cruz, L., Ryndock, E. J., & Meyers, C. (2014). Native human papillomavirus production, quantification, and infectivity analysis. In Cervical Cancer: Methods and Protocols (pp. 317-331). Springer New York. https://doi.org/10.1007/978-1-4939-2013-6_24