Natural human humoral response to salivary gland proteins of Anopheles mosquitoes in Thailand

Amornrat Waitayakul, Sangdao Somsri, Jetsumon Sattabongkot, Sornchai Looareesuwan, Liwang Cui, Rachanee Udomsangpetch

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

During blood feeding, arthropod vectors inject saliva into vertebrate hosts. The saliva is biochemically complex and pharmacologically active, and may play an important role in pathogen transmission. To examine whether mosquito saliva could elicit humoral immune response in humans under natural conditions, we have collected sera from malaria patients, healthy villagers, and people from a non-malarious region in Thailand. Here we have demonstrated that anti-Anopheles salivary protein antibodies occurred predominantly in patients with acute Plasmodium falciparum or P. vivax malaria, whereas people from a non-malarious area had no such antibodies. Besides, antibody levels against mosquito salivary proteins in malaria patients were highly variable, which may be related to the levels of mosquito exposure. Despite variability, patients' sera with high IgG titers consistently detected several proteins in Anopheles dirus salivary gland protein extracts. Immunohistochemical staining of Anopheles salivary glands with human sera showed that the salivary gland-specific IgGs reacted strongly with the median lobe. Comparison using Anopheles and Aedes salivary proteins suggests that the anti-salivary protein antibodies detected in malaria patients were Anopheles-specific, consistent with the major malaria vector status of An. dirus in this area.

Original languageEnglish (US)
Pages (from-to)66-73
Number of pages8
JournalActa Tropica
Volume98
Issue number1
DOIs
StatePublished - Apr 1 2006

Fingerprint

Salivary Proteins and Peptides
Anopheles
Thailand
salivary glands
Culicidae
humoral immunity
malaria
Malaria
Saliva
Anopheles dirus
blood serum
saliva
Antibodies
Salivary Glands
antibodies
proteins
Arthropod Vectors
Serum
Vivax Malaria
Infectious Disease Transmission

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Waitayakul, A., Somsri, S., Sattabongkot, J., Looareesuwan, S., Cui, L., & Udomsangpetch, R. (2006). Natural human humoral response to salivary gland proteins of Anopheles mosquitoes in Thailand. Acta Tropica, 98(1), 66-73. https://doi.org/10.1016/j.actatropica.2006.02.004
Waitayakul, Amornrat ; Somsri, Sangdao ; Sattabongkot, Jetsumon ; Looareesuwan, Sornchai ; Cui, Liwang ; Udomsangpetch, Rachanee. / Natural human humoral response to salivary gland proteins of Anopheles mosquitoes in Thailand. In: Acta Tropica. 2006 ; Vol. 98, No. 1. pp. 66-73.
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Waitayakul, A, Somsri, S, Sattabongkot, J, Looareesuwan, S, Cui, L & Udomsangpetch, R 2006, 'Natural human humoral response to salivary gland proteins of Anopheles mosquitoes in Thailand', Acta Tropica, vol. 98, no. 1, pp. 66-73. https://doi.org/10.1016/j.actatropica.2006.02.004

Natural human humoral response to salivary gland proteins of Anopheles mosquitoes in Thailand. / Waitayakul, Amornrat; Somsri, Sangdao; Sattabongkot, Jetsumon; Looareesuwan, Sornchai; Cui, Liwang; Udomsangpetch, Rachanee.

In: Acta Tropica, Vol. 98, No. 1, 01.04.2006, p. 66-73.

Research output: Contribution to journalArticle

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Waitayakul A, Somsri S, Sattabongkot J, Looareesuwan S, Cui L, Udomsangpetch R. Natural human humoral response to salivary gland proteins of Anopheles mosquitoes in Thailand. Acta Tropica. 2006 Apr 1;98(1):66-73. https://doi.org/10.1016/j.actatropica.2006.02.004