Necessity of the spacer peptide between CA and NC in the Rous sarcoma virus Gag protein

R. C. Craven, A. E. Leure-DuPree, C. R. Erdie, C. B. Wilson, J. W. Wills

Research output: Contribution to journalComment/debatepeer-review

68 Scopus citations

Abstract

A mutant of Rous sarcoma virus was constructed in which the nine amino acids that separate the CA and NC sequences in the Gag protein were deleted. The spacer peptide deletion mutant produced particles containing the normal complement of viral RNA and all of the viral proteins, including reverse transcriptase. Though electron microscopy revealed particles of normal morphology, the particles were noninfectious. The normally slow maturation of the CA protein, which involves cleavage of the spacer peptide from the carboxy terminus, was bypassed in this mutant, and the association between CA and the internal components of the core appears to have been disrupted. The results suggest that the spacer peptide has an essential role in directing folding and/or oligomerization of the CA subunits within the capsid structure.

Original languageEnglish (US)
Pages (from-to)6246-6252
Number of pages7
JournalJournal of virology
Volume67
Issue number10
DOIs
StatePublished - 1993

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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