Negative elongation factor (NELF) coordinates RNA polymerase II pausing, premature termination, and chromatin remodeling to regulate HIV transcription

Malini Natarajan, Gillian M. Schiralli Lester, Chanhyo Lee, Anamika Missra, Gregory A. Wasserman, Martin Steffen, David Scott Gilmour, Andrew J. Henderson

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Multiple mechanisms contribute to HIV latency, including NELF-mediated RNA polymerase II (RNAP II) pausing. Results: Paused RNAP II recruits a transcription termination factor and a transcriptional corepressor complex to the HIV promoter. Conclusion: PausedRNAPII couples premature transcription termination and chromatin remodeling to maintain HIV latency. Significance: Paused RNAP II may be targeted to purge latent HIV infection.

Original languageEnglish (US)
Pages (from-to)25995-26003
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number36
DOIs
StatePublished - Sep 6 2013

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Chromatin Assembly and Disassembly
RNA Polymerase II
Transcription
Chromatin
HIV
Co-Repressor Proteins
HIV Infections
Transcription Factors
negative elongation factor

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Natarajan, M., Schiralli Lester, G. M., Lee, C., Missra, A., Wasserman, G. A., Steffen, M., ... Henderson, A. J. (2013). Negative elongation factor (NELF) coordinates RNA polymerase II pausing, premature termination, and chromatin remodeling to regulate HIV transcription. Journal of Biological Chemistry, 288(36), 25995-26003. https://doi.org/10.1074/jbc.M113.496489
Natarajan, Malini ; Schiralli Lester, Gillian M. ; Lee, Chanhyo ; Missra, Anamika ; Wasserman, Gregory A. ; Steffen, Martin ; Gilmour, David Scott ; Henderson, Andrew J. / Negative elongation factor (NELF) coordinates RNA polymerase II pausing, premature termination, and chromatin remodeling to regulate HIV transcription. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 36. pp. 25995-26003.
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Negative elongation factor (NELF) coordinates RNA polymerase II pausing, premature termination, and chromatin remodeling to regulate HIV transcription. / Natarajan, Malini; Schiralli Lester, Gillian M.; Lee, Chanhyo; Missra, Anamika; Wasserman, Gregory A.; Steffen, Martin; Gilmour, David Scott; Henderson, Andrew J.

In: Journal of Biological Chemistry, Vol. 288, No. 36, 06.09.2013, p. 25995-26003.

Research output: Contribution to journalArticle

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AU - Natarajan, Malini

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AU - Gilmour, David Scott

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