TY - JOUR
T1 - Negligible Effect of Sodium Chloride on the Development and Function of TGF-β-Induced CD4 + Foxp3 + Regulatory T Cells
AU - Luo, Yang
AU - Xue, Youqiu
AU - Wang, Julie
AU - Dang, Junlong
AU - Fang, Qiannan
AU - Huang, Gonghua
AU - Olsen, Nancy
AU - Zheng, Song Guo
N1 - Funding Information:
This study was supported by grants from the National Key R&D Program of China ( 2017YFA0105801 to Y.L.), NIH ( R01 AR059103 , R61 AR073 409 , and NIH Star Award to S.G.Z.), the Zhujiang Innovative and Entrepreneurial Talent Team Award of Guangdong Province ( 2016 ZT 06S 252 to Y.L.), the National Natural Science Foundation of China ( 30972951 and 81671611 to Y.X.), the Natural Science Foundation of Guangdong Province ( 2014A030308005 to Q.F.), the Department of Science and Technology in Guangzhou City , and the Department of Science and Technology in Guangdong Province (to Q.F.).
Funding Information:
This study was supported by grants from the National Key R&D Program of China (2017YFA0105801 to Y.L.), NIH (R01 AR059103, R61 AR073 409, and NIH Star Award to S.G.Z.), the Zhujiang Innovative and Entrepreneurial Talent Team Award of Guangdong Province (2016 ZT 06S 252 to Y.L.), the National Natural Science Foundation of China (30972951 and 81671611 to Y.X.), the Natural Science Foundation of Guangdong Province (2014A030308005 to Q.F.), the Department of Science and Technology in Guangzhou City, and the Department of Science and Technology in Guangdong Province (to Q.F.).
Publisher Copyright:
© 2019 The Author(s)
PY - 2019/2/12
Y1 - 2019/2/12
N2 - High-salt diets inhibit the suppressive function of thymus-derived natural regulatory T cells (tTreg). Transforming growth factor β (TGF-β)-induced ex vivo regulatory T cells (iTreg) comprise another Treg subset that exhibits similarities and differences with tTreg. Here, we demonstrate that iTregs are completely stable and fully functional under high salt conditions. High salt does not influence the development, differentiation, and functional activities of iTreg but affects Foxp3 stability and function of tTreg in vitro and in vivo. In addition, high salt does not significantly change the transcription profiles of the iTreg signature or pro-inflammatory genes. Therefore, we conclude that iTreg, unlike tTreg, are stable and functional in the presence of high salt. Our findings provide additional evidence that iTreg may have different biological features from tTreg and suggest a greater potential for clinical utility in patients with autoimmune diseases, in which the complicated role of environmental factors, including diet, must be considered.
AB - High-salt diets inhibit the suppressive function of thymus-derived natural regulatory T cells (tTreg). Transforming growth factor β (TGF-β)-induced ex vivo regulatory T cells (iTreg) comprise another Treg subset that exhibits similarities and differences with tTreg. Here, we demonstrate that iTregs are completely stable and fully functional under high salt conditions. High salt does not influence the development, differentiation, and functional activities of iTreg but affects Foxp3 stability and function of tTreg in vitro and in vivo. In addition, high salt does not significantly change the transcription profiles of the iTreg signature or pro-inflammatory genes. Therefore, we conclude that iTreg, unlike tTreg, are stable and functional in the presence of high salt. Our findings provide additional evidence that iTreg may have different biological features from tTreg and suggest a greater potential for clinical utility in patients with autoimmune diseases, in which the complicated role of environmental factors, including diet, must be considered.
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U2 - 10.1016/j.celrep.2019.01.066
DO - 10.1016/j.celrep.2019.01.066
M3 - Article
C2 - 30759396
AN - SCOPUS:85061001812
VL - 26
SP - 1869-1879.e3
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 7
ER -