Nestin reporter transgene labels multiple central nervous system precursor cells

Francis G. Szele, Avery S. Walker, Gwendolyn E. Goings, Yongsoo Kim, Richard J. Miller, Anjen Chenn

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Embryonic neuroepithelia and adult subventricular zone (SVZ) stem and progenitor cells express nestin. We characterized a transgenic line that expresses enhanced green fluorescent protein (eGFP) specified to neural tissue by the second intronic enhancer of the nestin promoter that had several novel features. During embryogenesis, the dorsal telencephalon contained many and the ventral telencephalon few eGFP+ cells. eGFP+ cells were found in postnatal and adult neurogenic regions. eGFP+ cells in the SVZ expressed multiple phenotype markers, glial fibrillary acidic protein, Dlx, and neuroblast-specific molecules suggesting the transgene is expressed through the lineage. eGFP+ cell numbers increased in the SVZ after cortical injury, suggesting this line will be useful in probing postinjury neurogenesis. In non-neurogenic regions, eGFP was strongly expressed in oligodendrocyte progenitors, but not in astrocytes, even when they were reactive. This eGFP+ mouse will facilitate studies of proliferative neuroepithelia and adult neurogenesis, as well as of parenchymal oligodendrocytes.

Original languageEnglish (US)
Article number894374
JournalNeural Plasticity
Volume2010
DOIs
StatePublished - Jan 1 2010

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Nestin
Transgenes
Central Nervous System
Lateral Ventricles
Telencephalon
Neurogenesis
Oligodendroglia
Stem Cells
Glial Fibrillary Acidic Protein
enhanced green fluorescent protein
Astrocytes
Embryonic Development
Cell Count
Phenotype
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Szele, F. G., Walker, A. S., Goings, G. E., Kim, Y., Miller, R. J., & Chenn, A. (2010). Nestin reporter transgene labels multiple central nervous system precursor cells. Neural Plasticity, 2010, [894374]. https://doi.org/10.1155/2010/894374
Szele, Francis G. ; Walker, Avery S. ; Goings, Gwendolyn E. ; Kim, Yongsoo ; Miller, Richard J. ; Chenn, Anjen. / Nestin reporter transgene labels multiple central nervous system precursor cells. In: Neural Plasticity. 2010 ; Vol. 2010.
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Nestin reporter transgene labels multiple central nervous system precursor cells. / Szele, Francis G.; Walker, Avery S.; Goings, Gwendolyn E.; Kim, Yongsoo; Miller, Richard J.; Chenn, Anjen.

In: Neural Plasticity, Vol. 2010, 894374, 01.01.2010.

Research output: Contribution to journalArticle

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