Network of coupled promoting motions in enzyme catalysis

Pratul K. Agarwal, Salomon R. Billeter, P. T.Ravi Rajagopalan, Stephen J. Benkovic, Sharon Hammes-Schiffer

Research output: Contribution to journalArticle

375 Scopus citations

Abstract

A network of coupled promoting motions in the enzyme dihydrofolate reductase is identified and characterized. The present identification is based on genomic analysis for sequence conservation, kinetic measurements of multiple mutations, and mixed quantum/classical molecular dynamics simulations of hydride transfer. The motions in this network span time scales of femtoseconds to milliseconds and are found on the exterior of the enzyme as well as in the active site. This type of network has broad implications for an expanded role of the protein fold in catalysis as well as ancillaries such as the engineering of altered protein function and the action of drugs distal to the active site.

Original languageEnglish (US)
Pages (from-to)2794-2799
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number5
DOIs
StatePublished - Mar 5 2002

All Science Journal Classification (ASJC) codes

  • General

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    Agarwal, P. K., Billeter, S. R., Rajagopalan, P. T. R., Benkovic, S. J., & Hammes-Schiffer, S. (2002). Network of coupled promoting motions in enzyme catalysis. Proceedings of the National Academy of Sciences of the United States of America, 99(5), 2794-2799. https://doi.org/10.1073/pnas.052005999