Neuroblastoma biosensor system

R. Silva, R. Gaumond, E. W. Barrett, M. V. Phelps, H. R. Allcock, N. Vasudevan

Research output: Contribution to journalConference articlepeer-review

Abstract

SKN-B-E(2)C neuroblastoma cells were cultured on poly[(methoxyethoxyethoxy) 1.0(cinammyloxy)1.0] phosphazene coated indium-tin-oxide (ITO) glass slides for a period of 35 days. Electrical activity was recorded periodically using a custom fabricated flow chamber. At 35-days of culture, "pacemaker" field potentials of 30 microvolts, 1-4 sec-1 rate were measured from the cell ensemble. The mean inter spike interval (M isi for the basal firing rate was measured at 1.5±0.1 sec. Using the flow chamber, the neuroblastoma were stimulated with 0.05M solution of monosodium glutamate and the neuroelectrical response was recorded. The M isi for the stimulated cells increased to 0.57±0.1 sec. After rinsing away the monosodium glutamate with fresh culture media, the summed cell firing rate recovered to a baseline rate of 1.5±0.1 sec. Capsaicin was introduced as a mild nenrotoxin and was shown to disrupt the uniform firing pattern seen under basal activity. The Misi for the capsaicin response was 1.5±0.6 sec. The original signal was recovered after rinsing off the capsaicin. Introduction of a 1μM tetrodotoxin solution extinguished the field potential waveform.

Original languageEnglish (US)
Pages (from-to)110-111
Number of pages2
JournalProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
Volume30
StatePublished - 2004
EventProceedings of the IEEE 30th Annual Northeast Bioengineering Conference - Springfield, MA, United States
Duration: Apr 17 2004Apr 18 2004

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)

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