NeuroD1 overexpression in spinal neurons accelerates axonal regeneration after sciatic nerve injury

Muhua Lai, Mengjie Pan, Longjiao Ge, Jingmin Liu, Junyao Deng, Xianghai Wang, Lixia Li, Jinkun Wen, Dandan Tan, Haowen Zhang, Xiaofang Hu, Lanya Fu, Yizhou Xu, Zhenlin Li, Xiaozhong Qiu, Gong Chen, Jiasong Guo

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1 Scopus citations

Abstract

Neurogenic differentiation 1 (NeuroD1) is mainlyexpressed in developing neurons where it plays critical roles in neuronal maturation and neurite elongation. The potential role and mechanism of NeuroD1 in adult axonal regeneration is not clear. The present study used synapsin (SYN) Cre and AAV9-Flex vectors to conditionally overexpress NeuroD1 in adult spinal neurons and found that NeuroD1 overexpression significantly accelerated axonal regeneration and functional recovery after sciatic nerve injury. Further in vitro and in vivo experiments suggested that the mechanism of NeuroD1 promotion on axonal regeneration was related to its regulation of the expression of neurotrophin BDNF and its receptor TrkB as well as a microtubule severing protein spastin.

Original languageEnglish (US)
Article number113215
JournalExperimental Neurology
Volume327
DOIs
StatePublished - May 2020

All Science Journal Classification (ASJC) codes

  • Neurology
  • Developmental Neuroscience

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